Brain-derived neurotrophic factor is upregulated in rats with chronic pancreatitis and mediates pain behavior

Michael S. Hughes, Mohan Shenoy, Lian Sheng Liu, Tugba Colak, Kshama Mehta, Pankaj Jay Pasricha

Research output: Contribution to journalArticle

Abstract

Objectives: We examined the role of brain-derived neurotrophic factor (BDNF) in the pathogenesis of pain in an experimental model of chronic pancreatitis (CP). Methods: Pancreatitis was induced by retrograde infusion of trinitrobenzene sulfonic acid into the pancreatic duct of adult rats. Twenty-one days after injection, BDNF expression was examined in pancreas-specific dorsal root ganglia (DRGs) by immunohistochemistry, and protein levels were quantified from DRGs and spinal cord extracts. The effects of intrathecal infusion of a neutralizing antibody to BDNF on pancreatic hyperalgesia were assessed by the sensitivity of the abdominal wall to filament probing as well as the nocifensive behavior to electrical stimulation of the pancreas. Results: Levels of BDNF in DRGs and spinal cords (T9-13) were significantly higher in trinitrobenzene sulfonic acid rats compared with controls, accompanied by an increase in the number of pancreas-specific neurons expressing BDNF immunoreactivity. Brain-derived neurotrophic factor antagonism suppressed phospho-tropomyosin- related kinase B receptor levels in the spinal cord and significantly reduced behavioral responses in rats with CP. Conclusions: Brain-derived neurotrophic factor is upregulated in pancreas-specific primary afferent neurons in rats with CP, and BDNF antagonism is associated with a reduction of pain-related behavior in these animals, suggesting an important role for this neurotransmitter in the nociception of CP.

Original languageEnglish (US)
Pages (from-to)551-556
Number of pages6
JournalPancreas
Volume40
Issue number4
DOIs
StatePublished - May 2011
Externally publishedYes

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Brain-Derived Neurotrophic Factor
Chronic Pancreatitis
Pain
Pancreas
Spinal Ganglia
Trinitrobenzenes
Spinal Cord
Sulfonic Acids
Afferent Neurons
Animal Behavior
Nociception
Pancreatic Ducts
Hyperalgesia
Abdominal Wall
Neutralizing Antibodies
Pancreatitis
Electric Stimulation
Neurotransmitter Agents
Theoretical Models
Immunohistochemistry

Keywords

  • BDNF
  • Chronic pancreatitis
  • sensory neurons
  • TrkB
  • visceral pain

ASJC Scopus subject areas

  • Hepatology
  • Internal Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Brain-derived neurotrophic factor is upregulated in rats with chronic pancreatitis and mediates pain behavior. / Hughes, Michael S.; Shenoy, Mohan; Liu, Lian Sheng; Colak, Tugba; Mehta, Kshama; Pasricha, Pankaj Jay.

In: Pancreas, Vol. 40, No. 4, 05.2011, p. 551-556.

Research output: Contribution to journalArticle

Hughes, Michael S. ; Shenoy, Mohan ; Liu, Lian Sheng ; Colak, Tugba ; Mehta, Kshama ; Pasricha, Pankaj Jay. / Brain-derived neurotrophic factor is upregulated in rats with chronic pancreatitis and mediates pain behavior. In: Pancreas. 2011 ; Vol. 40, No. 4. pp. 551-556.
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