Brain adducin: A protein kinase C substrate that may mediate site-directed assembly at the spectrin-actin junction

V. Bennett, K. Gardner, J. P. Steiner

Research output: Contribution to journalArticle

Abstract

Erythrocyte adducin is a membrane skeletal protein that binds to calmodulin, is a major substrate for protein kinase C, and associates preferentially with spectrin-actin complexes. Erythrocyte adducin also promotes association of spectrin with actin, and this activity is inhibited by calmodulin. This study describes the isolation and characterization of a brain peripheral membrane protein closely related to erythrocyte adducin. Brain and erythrocyte adducin have at least 50% antigenic sites in common, each contains a protease-resistant core of M(r) = 48,000-48,500, and both proteins are comprised of two partially homologous polypeptides of M(r) = 103,000 and 97,000 (erythrocytes) and M(r) = 104,000 and 107,000 - 110,000 (brain). Brain and erythrocyte adducin associate preferentially with spectrin-actin complexes as compared to spectrin or actin alone, and both proteins also promote binding of spectrin to actin. Brain adducin binds calmodulin in a calcium-dependent manner, although the K(d) of 1.3 μM is weaker by 5-6-fold than the K(d) of erythrocyte adducin for calmodulin. Brain adducin is a substrate for protein kinase C in vitro and can accept up to 2 mol of phosphate/mol of protein. Adducin provides a potential mechanism in cells for mediating site-directed assembly of additional spectrin molecules and possibly other proteins at the spectrin-actin junction. Brain tissue contains 12 pmol of adducin/mg of membrane protein, which is the most of any tissue examined other than erythrocytes, which have 50 pmol/mg. The presence of high amounts of adducin in brain suggests some role for this protein in specialized activities of nerve cells.

Original languageEnglish (US)
Pages (from-to)5860-5869
Number of pages10
JournalJournal of Biological Chemistry
Volume263
Issue number12
StatePublished - 1988
Externally publishedYes

Fingerprint

Spectrin
Protein Kinase C
Actins
Brain
Erythrocytes
Substrates
Calmodulin
Membrane Proteins
Proteins
adducin
Tissue
Neurons
Peptide Hydrolases
Phosphates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Brain adducin : A protein kinase C substrate that may mediate site-directed assembly at the spectrin-actin junction. / Bennett, V.; Gardner, K.; Steiner, J. P.

In: Journal of Biological Chemistry, Vol. 263, No. 12, 1988, p. 5860-5869.

Research output: Contribution to journalArticle

@article{05543855450f46b9b2548343b58ab036,
title = "Brain adducin: A protein kinase C substrate that may mediate site-directed assembly at the spectrin-actin junction",
abstract = "Erythrocyte adducin is a membrane skeletal protein that binds to calmodulin, is a major substrate for protein kinase C, and associates preferentially with spectrin-actin complexes. Erythrocyte adducin also promotes association of spectrin with actin, and this activity is inhibited by calmodulin. This study describes the isolation and characterization of a brain peripheral membrane protein closely related to erythrocyte adducin. Brain and erythrocyte adducin have at least 50{\%} antigenic sites in common, each contains a protease-resistant core of M(r) = 48,000-48,500, and both proteins are comprised of two partially homologous polypeptides of M(r) = 103,000 and 97,000 (erythrocytes) and M(r) = 104,000 and 107,000 - 110,000 (brain). Brain and erythrocyte adducin associate preferentially with spectrin-actin complexes as compared to spectrin or actin alone, and both proteins also promote binding of spectrin to actin. Brain adducin binds calmodulin in a calcium-dependent manner, although the K(d) of 1.3 μM is weaker by 5-6-fold than the K(d) of erythrocyte adducin for calmodulin. Brain adducin is a substrate for protein kinase C in vitro and can accept up to 2 mol of phosphate/mol of protein. Adducin provides a potential mechanism in cells for mediating site-directed assembly of additional spectrin molecules and possibly other proteins at the spectrin-actin junction. Brain tissue contains 12 pmol of adducin/mg of membrane protein, which is the most of any tissue examined other than erythrocytes, which have 50 pmol/mg. The presence of high amounts of adducin in brain suggests some role for this protein in specialized activities of nerve cells.",
author = "V. Bennett and K. Gardner and Steiner, {J. P.}",
year = "1988",
language = "English (US)",
volume = "263",
pages = "5860--5869",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "12",

}

TY - JOUR

T1 - Brain adducin

T2 - A protein kinase C substrate that may mediate site-directed assembly at the spectrin-actin junction

AU - Bennett, V.

AU - Gardner, K.

AU - Steiner, J. P.

PY - 1988

Y1 - 1988

N2 - Erythrocyte adducin is a membrane skeletal protein that binds to calmodulin, is a major substrate for protein kinase C, and associates preferentially with spectrin-actin complexes. Erythrocyte adducin also promotes association of spectrin with actin, and this activity is inhibited by calmodulin. This study describes the isolation and characterization of a brain peripheral membrane protein closely related to erythrocyte adducin. Brain and erythrocyte adducin have at least 50% antigenic sites in common, each contains a protease-resistant core of M(r) = 48,000-48,500, and both proteins are comprised of two partially homologous polypeptides of M(r) = 103,000 and 97,000 (erythrocytes) and M(r) = 104,000 and 107,000 - 110,000 (brain). Brain and erythrocyte adducin associate preferentially with spectrin-actin complexes as compared to spectrin or actin alone, and both proteins also promote binding of spectrin to actin. Brain adducin binds calmodulin in a calcium-dependent manner, although the K(d) of 1.3 μM is weaker by 5-6-fold than the K(d) of erythrocyte adducin for calmodulin. Brain adducin is a substrate for protein kinase C in vitro and can accept up to 2 mol of phosphate/mol of protein. Adducin provides a potential mechanism in cells for mediating site-directed assembly of additional spectrin molecules and possibly other proteins at the spectrin-actin junction. Brain tissue contains 12 pmol of adducin/mg of membrane protein, which is the most of any tissue examined other than erythrocytes, which have 50 pmol/mg. The presence of high amounts of adducin in brain suggests some role for this protein in specialized activities of nerve cells.

AB - Erythrocyte adducin is a membrane skeletal protein that binds to calmodulin, is a major substrate for protein kinase C, and associates preferentially with spectrin-actin complexes. Erythrocyte adducin also promotes association of spectrin with actin, and this activity is inhibited by calmodulin. This study describes the isolation and characterization of a brain peripheral membrane protein closely related to erythrocyte adducin. Brain and erythrocyte adducin have at least 50% antigenic sites in common, each contains a protease-resistant core of M(r) = 48,000-48,500, and both proteins are comprised of two partially homologous polypeptides of M(r) = 103,000 and 97,000 (erythrocytes) and M(r) = 104,000 and 107,000 - 110,000 (brain). Brain and erythrocyte adducin associate preferentially with spectrin-actin complexes as compared to spectrin or actin alone, and both proteins also promote binding of spectrin to actin. Brain adducin binds calmodulin in a calcium-dependent manner, although the K(d) of 1.3 μM is weaker by 5-6-fold than the K(d) of erythrocyte adducin for calmodulin. Brain adducin is a substrate for protein kinase C in vitro and can accept up to 2 mol of phosphate/mol of protein. Adducin provides a potential mechanism in cells for mediating site-directed assembly of additional spectrin molecules and possibly other proteins at the spectrin-actin junction. Brain tissue contains 12 pmol of adducin/mg of membrane protein, which is the most of any tissue examined other than erythrocytes, which have 50 pmol/mg. The presence of high amounts of adducin in brain suggests some role for this protein in specialized activities of nerve cells.

UR - http://www.scopus.com/inward/record.url?scp=0023880432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023880432&partnerID=8YFLogxK

M3 - Article

C2 - 2451672

AN - SCOPUS:0023880432

VL - 263

SP - 5860

EP - 5869

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 12

ER -