Bradykinin receptors localized by quantitative autoradiography in kidney, ureter, and bladder

D. C. Manning, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

We have localized high affinity [3H]bradykinin receptor binding sites by in vitro autoradiography in kidney, ureter, and bladder of the guinea pig. The peptide pharmacology of the binding sites corresponds to that of high affinity physiological bradykinin receptors previously described (Manning, D.C., R. Vavrek, J.M. Stewart, and S.H. Snyder. J. Pharmacol. Exp. Ther. 237: 504-512, 1986). In the kidney, receptors are concentrated in the medulla with negligible binding in the cortex. Medullary receptors are localized to the interstitium just beneath the basal membrane of collecting tubule cells and between tubules. In the ureter and bladder, receptors are confined to the lamina propria just beneath the epithelial layer. Localizations in the kidney may relate to the diuretic and natriuretic actions of bradykinin. Ureteral and bladder receptors may be associated with a role of bradykinin in pain and inflammation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume256
Issue number5
StatePublished - 1989

Fingerprint

Bradykinin Receptors
Ureter
Autoradiography
Urinary Bladder
Bradykinin
Kidney
Binding Sites
Diuretics
Guinea Pigs
Mucous Membrane
Pharmacology
Inflammation
Pain
Peptides
Membranes

ASJC Scopus subject areas

  • Physiology

Cite this

@article{1443ea498c5a4fac8238cde32139eb97,
title = "Bradykinin receptors localized by quantitative autoradiography in kidney, ureter, and bladder",
abstract = "We have localized high affinity [3H]bradykinin receptor binding sites by in vitro autoradiography in kidney, ureter, and bladder of the guinea pig. The peptide pharmacology of the binding sites corresponds to that of high affinity physiological bradykinin receptors previously described (Manning, D.C., R. Vavrek, J.M. Stewart, and S.H. Snyder. J. Pharmacol. Exp. Ther. 237: 504-512, 1986). In the kidney, receptors are concentrated in the medulla with negligible binding in the cortex. Medullary receptors are localized to the interstitium just beneath the basal membrane of collecting tubule cells and between tubules. In the ureter and bladder, receptors are confined to the lamina propria just beneath the epithelial layer. Localizations in the kidney may relate to the diuretic and natriuretic actions of bradykinin. Ureteral and bladder receptors may be associated with a role of bradykinin in pain and inflammation.",
author = "Manning, {D. C.} and Snyder, {Solomon H}",
year = "1989",
language = "English (US)",
volume = "256",
journal = "American Journal of Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "5",

}

TY - JOUR

T1 - Bradykinin receptors localized by quantitative autoradiography in kidney, ureter, and bladder

AU - Manning, D. C.

AU - Snyder, Solomon H

PY - 1989

Y1 - 1989

N2 - We have localized high affinity [3H]bradykinin receptor binding sites by in vitro autoradiography in kidney, ureter, and bladder of the guinea pig. The peptide pharmacology of the binding sites corresponds to that of high affinity physiological bradykinin receptors previously described (Manning, D.C., R. Vavrek, J.M. Stewart, and S.H. Snyder. J. Pharmacol. Exp. Ther. 237: 504-512, 1986). In the kidney, receptors are concentrated in the medulla with negligible binding in the cortex. Medullary receptors are localized to the interstitium just beneath the basal membrane of collecting tubule cells and between tubules. In the ureter and bladder, receptors are confined to the lamina propria just beneath the epithelial layer. Localizations in the kidney may relate to the diuretic and natriuretic actions of bradykinin. Ureteral and bladder receptors may be associated with a role of bradykinin in pain and inflammation.

AB - We have localized high affinity [3H]bradykinin receptor binding sites by in vitro autoradiography in kidney, ureter, and bladder of the guinea pig. The peptide pharmacology of the binding sites corresponds to that of high affinity physiological bradykinin receptors previously described (Manning, D.C., R. Vavrek, J.M. Stewart, and S.H. Snyder. J. Pharmacol. Exp. Ther. 237: 504-512, 1986). In the kidney, receptors are concentrated in the medulla with negligible binding in the cortex. Medullary receptors are localized to the interstitium just beneath the basal membrane of collecting tubule cells and between tubules. In the ureter and bladder, receptors are confined to the lamina propria just beneath the epithelial layer. Localizations in the kidney may relate to the diuretic and natriuretic actions of bradykinin. Ureteral and bladder receptors may be associated with a role of bradykinin in pain and inflammation.

UR - http://www.scopus.com/inward/record.url?scp=0024359603&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024359603&partnerID=8YFLogxK

M3 - Article

VL - 256

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6135

IS - 5

ER -