Abstract
Telomerase is a DNA polymerase fundamental to the replication and maintenance of telomere sequences at chromosome ends. The RNA component of telomerase is essential for the synthesis of telomere repeats. In vitro, the template domain (5′-CAACCCCAA-3′) of the Tetrahymena telomerase RNA dictates the addition of Tetrahymena-specific telomere repeats d(TTGGGG)n, onto the 3′ end of G-rich or telomeric substrates that are base-paired with the template and alignment regions of the RNA. Using a reconstituted in vitro system, we determined that altering the sequence of the alignment and template domains affects processivity of telomerase without abolishing telomerase activity. These results suggest that alternative template/alignment regions may be functional. In the ciliate telomerase RNAs, there is a conserved sequence 5′-(CU)GUCA-3′, located two residues upstream of the template domain. The location and sequence of this conserved domain defined the 5′ boundary of the template region. These data provide insights into the regulation of telomere synthesis by telomerase.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2227-2239 |
| Number of pages | 13 |
| Journal | Genes & development |
| Volume | 9 |
| Issue number | 18 |
| State | Published - Sep 15 1995 |
| Externally published | Yes |
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Keywords
- Polymerase
- Ribonucleoprotein
- Telomerase
- Telomere
- Tetrahymena
ASJC Scopus subject areas
- Developmental Biology
- Genetics
Cite this
Boundary elements of the Tetrahymena telomerase RNA template and alignment domains. / Autexier, Chantal; Greider, Carol W.
In: Genes & development, Vol. 9, No. 18, 15.09.1995, p. 2227-2239.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Boundary elements of the Tetrahymena telomerase RNA template and alignment domains
AU - Autexier, Chantal
AU - Greider, Carol W
PY - 1995/9/15
Y1 - 1995/9/15
N2 - Telomerase is a DNA polymerase fundamental to the replication and maintenance of telomere sequences at chromosome ends. The RNA component of telomerase is essential for the synthesis of telomere repeats. In vitro, the template domain (5′-CAACCCCAA-3′) of the Tetrahymena telomerase RNA dictates the addition of Tetrahymena-specific telomere repeats d(TTGGGG)n, onto the 3′ end of G-rich or telomeric substrates that are base-paired with the template and alignment regions of the RNA. Using a reconstituted in vitro system, we determined that altering the sequence of the alignment and template domains affects processivity of telomerase without abolishing telomerase activity. These results suggest that alternative template/alignment regions may be functional. In the ciliate telomerase RNAs, there is a conserved sequence 5′-(CU)GUCA-3′, located two residues upstream of the template domain. The location and sequence of this conserved domain defined the 5′ boundary of the template region. These data provide insights into the regulation of telomere synthesis by telomerase.
AB - Telomerase is a DNA polymerase fundamental to the replication and maintenance of telomere sequences at chromosome ends. The RNA component of telomerase is essential for the synthesis of telomere repeats. In vitro, the template domain (5′-CAACCCCAA-3′) of the Tetrahymena telomerase RNA dictates the addition of Tetrahymena-specific telomere repeats d(TTGGGG)n, onto the 3′ end of G-rich or telomeric substrates that are base-paired with the template and alignment regions of the RNA. Using a reconstituted in vitro system, we determined that altering the sequence of the alignment and template domains affects processivity of telomerase without abolishing telomerase activity. These results suggest that alternative template/alignment regions may be functional. In the ciliate telomerase RNAs, there is a conserved sequence 5′-(CU)GUCA-3′, located two residues upstream of the template domain. The location and sequence of this conserved domain defined the 5′ boundary of the template region. These data provide insights into the regulation of telomere synthesis by telomerase.
KW - Polymerase
KW - Ribonucleoprotein
KW - Telomerase
KW - Telomere
KW - Tetrahymena
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UR - http://www.scopus.com/inward/citedby.url?scp=0029128539&partnerID=8YFLogxK
M3 - Article
C2 - 7557377
AN - SCOPUS:0029128539
VL - 9
SP - 2227
EP - 2239
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 18
ER -