TY - JOUR
T1 - Boron dipyrromethene fluorophore based fluorescence sensor for the selective imaging of Zn(II) in living cells
AU - Wu, Yunkou
AU - Peng, Xiaojun
AU - Guo, Binchen
AU - Fan, Jiangli
AU - Zhang, Zhichao
AU - Wang, Jingyun
AU - Cui, Aijun
AU - Gao, Yunling
PY - 2005/4/21
Y1 - 2005/4/21
N2 - A simple PET fluorescence sensor (BDA) for Zn2- that utilizes 1,3,5,7-tetramethyl-boron dipyrromethene as a reporting group and di(2-picolyl)amine as a chelator for Zn2+ has been synthesized and characterized. BDA has an excitation (491 nm) and emission wavelength (509 nm) in the visible range. The fluorescence quantum yields of the zinc-free and zinc-bound states of BDA are 0.077 and 0.857, respectively. With a low pK a of 2.1 ± 0.1, BDA has the advantage of less sensitivity to pH than fluorescein-based Zn2+ sensors, and the fluorescence emission of zinc-binding is pH-independent in the range of pH 3-10. Under physiological conditions, metal ions such as Na+ K-, Ca2+:, Mg2+, Mn2+ and Fe2+ have little interference. The apparent dissociation constant (Kd) is 1.0 ± 0.1 nM. Using fluorescence microscopy, the sensor is shown to be capable of imaging intracellular Zn2+ changes.
AB - A simple PET fluorescence sensor (BDA) for Zn2- that utilizes 1,3,5,7-tetramethyl-boron dipyrromethene as a reporting group and di(2-picolyl)amine as a chelator for Zn2+ has been synthesized and characterized. BDA has an excitation (491 nm) and emission wavelength (509 nm) in the visible range. The fluorescence quantum yields of the zinc-free and zinc-bound states of BDA are 0.077 and 0.857, respectively. With a low pK a of 2.1 ± 0.1, BDA has the advantage of less sensitivity to pH than fluorescein-based Zn2+ sensors, and the fluorescence emission of zinc-binding is pH-independent in the range of pH 3-10. Under physiological conditions, metal ions such as Na+ K-, Ca2+:, Mg2+, Mn2+ and Fe2+ have little interference. The apparent dissociation constant (Kd) is 1.0 ± 0.1 nM. Using fluorescence microscopy, the sensor is shown to be capable of imaging intracellular Zn2+ changes.
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U2 - 10.1039/b501795e
DO - 10.1039/b501795e
M3 - Article
C2 - 15827633
AN - SCOPUS:18444364198
SN - 1477-0520
VL - 3
SP - 1387
EP - 1392
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
IS - 8
ER -