Borna disease virus in peripheral blood mononuclear and bone marrow cells of neonatally and chronically infected rats

A. M. Sierra-Honigmann, S. A. Rubin, M. G. Estafanous, Robert H Yolken, K. M. Carbone

Research output: Contribution to journalArticle

Abstract

Borna disease virus (BDV) establishes a persistent infection in cells of the nervous system in rats. The response, or lack thereof, of the immune system to BDV infection of neurons is responsible for the presence or absence, respectively, of Borna disease. We recently demonstrated transmission of BDV by bone marrow cells from neonatally infected rats. Our findings suggested the possibility of a heretofore unsuspected interaction between BDV and the immune system, that of direct effects of BDV infection on the cells of the immune system. This report enlarges upon the previous findings and confirms the presence of BDV RNA in bone marrow cells of neonatally infected rats, using a reverse transcription-polymerization chain reaction-enzyme immunosorbent assay (RT-PCR-EIA). In addition, we detected BDV RNA in peripheral blood mononuclear cells of neonatally infected rats, and in rats inoculated as adults in the chronic, but not the acute, stage of infection. In addition, the RT-PCR-EIA technique identified BDV RNA in cerebrospinal fluid, nasal secretions, saliva, urine and stool. BDV-sequences were not detected in the plasma of infected animals nor in the body fluids and tissues of normal rats.

Original languageEnglish (US)
Pages (from-to)31-36
Number of pages6
JournalJournal of Neuroimmunology
Volume45
Issue number1-2
DOIs
StatePublished - 1993

Fingerprint

Borna disease virus
Bone Marrow Cells
Enzyme Assays
Virus Diseases
RNA
Polymerization
Reverse Transcription
Borna Disease
Immune System
Immunosorbent Techniques
Fluids and Secretions
Immunosorbents
Immune System Diseases
Body Fluids
Infection
Nose
Saliva
Nervous System
Cerebrospinal Fluid
Blood Cells

Keywords

  • Bone marrow
  • Borna disease virus
  • Encephalitis
  • Mononuclear cells
  • PCR
  • Rat

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

Borna disease virus in peripheral blood mononuclear and bone marrow cells of neonatally and chronically infected rats. / Sierra-Honigmann, A. M.; Rubin, S. A.; Estafanous, M. G.; Yolken, Robert H; Carbone, K. M.

In: Journal of Neuroimmunology, Vol. 45, No. 1-2, 1993, p. 31-36.

Research output: Contribution to journalArticle

Sierra-Honigmann, A. M. ; Rubin, S. A. ; Estafanous, M. G. ; Yolken, Robert H ; Carbone, K. M. / Borna disease virus in peripheral blood mononuclear and bone marrow cells of neonatally and chronically infected rats. In: Journal of Neuroimmunology. 1993 ; Vol. 45, No. 1-2. pp. 31-36.
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