TY - JOUR
T1 - Borna disease virus in peripheral blood mononuclear and bone marrow cells of neonatally and chronically infected rats
AU - Sierra-Honigmann, A. M.
AU - Rubin, S. A.
AU - Estafanous, M. G.
AU - Yolken, R. H.
AU - Carbone, K. M.
N1 - Funding Information:
This work was supported by NIH Grant NS289599 (K.M.C.) and NIMH Grant MH48948 (K.M.C., A.S.H.), the Stanley Foundation for Research (A.S.H.) and the Howard Hughes Foundation (M.G.E.). We thank W. Ian Lipkin, of the University of California at Irvine, CA, for providing us with the sequence of BDV-clone AB5, Drs. Diane E. Griffin and W. Ian Lipkin for helpful discussions, and Dr. Robert Carrioco for providing the monoclonal antibody directed against RNA/DNA hybrids.
PY - 1993/6
Y1 - 1993/6
N2 - Borna disease virus (BDV) establishes a persistent infection in cells of the nervous system in rats. The response, or lack thereof, of the immune system to BDV infection of neurons is responsible for the presence or absence, respectively, of Borna disease. We recently demonstrated transmission of BDV by bone marrow cells from neonatally infected rats. Our findings suggested the possibility of a heretofore unsuspected interaction between BDV and the immune system, that of direct effects of BDV infection on the cells of the immune system. This report enlarges upon the previous findings and confirms the presence of BDV RNA in bone marrow cells of neonatally infected rats, using a reverse transcription-polymerization chain reaction-enzyme immunosorbent assay (RT-PCR-EIA). In addition, we detected BDV RNA in peripheral blood mononuclear cells of neonatally infected rats, and in rats inoculated as adults in the chronic, but not the acute, stage of infection. In addition, the RT-PCR-EIA technique identified BDV RNA in cerebrospinal fluid, nasal secretions, saliva, urine and stool. BDV-sequences were not detected in the plasma of infected animals nor in the body fluids and tissues of normal rats.
AB - Borna disease virus (BDV) establishes a persistent infection in cells of the nervous system in rats. The response, or lack thereof, of the immune system to BDV infection of neurons is responsible for the presence or absence, respectively, of Borna disease. We recently demonstrated transmission of BDV by bone marrow cells from neonatally infected rats. Our findings suggested the possibility of a heretofore unsuspected interaction between BDV and the immune system, that of direct effects of BDV infection on the cells of the immune system. This report enlarges upon the previous findings and confirms the presence of BDV RNA in bone marrow cells of neonatally infected rats, using a reverse transcription-polymerization chain reaction-enzyme immunosorbent assay (RT-PCR-EIA). In addition, we detected BDV RNA in peripheral blood mononuclear cells of neonatally infected rats, and in rats inoculated as adults in the chronic, but not the acute, stage of infection. In addition, the RT-PCR-EIA technique identified BDV RNA in cerebrospinal fluid, nasal secretions, saliva, urine and stool. BDV-sequences were not detected in the plasma of infected animals nor in the body fluids and tissues of normal rats.
KW - Bone marrow
KW - Borna disease virus
KW - Encephalitis
KW - Mononuclear cells
KW - PCR
KW - Rat
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U2 - 10.1016/0165-5728(93)90160-Z
DO - 10.1016/0165-5728(93)90160-Z
M3 - Article
C2 - 8331163
AN - SCOPUS:0027178804
SN - 0165-5728
VL - 45
SP - 31
EP - 36
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
IS - 1-2
ER -