BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma

Dmitri I. Loukinov, Elena Pugacheva, Sergei Vatolin, Svetlan D. Pack, Hanlim Moon, Igor Chernukhin, Poonam Mannan, Erik Larsson, Chandrasekhar Kanduri, Alexander A. Vostrov, Hengmi Cui, Emily L. Niemitz, John E.J. Rasko, France M. Docquier, Malathi Kistler, Joseph J. Breen, Zhengping Zhuang, Wolfgang W. Quitschke, Rainer Renkawitz, Elena M. KlenovaAndrew P. Feinberg, Rolf Ohlsson, Herbert C. Morse, Victor V. Lobanenkov

Research output: Contribution to journalArticlepeer-review

Abstract

CTCF, a conserved, ubiquitous, and highly versatile 11-zinc-finger factor involved in various aspects of gene regulation, forms methylation-sensitive insulators that regulate X chromosome inactivation and expression of imprinted genes. We document here the existence of a paralogous gene with the same exons encoding the 11-zinc-finger domain as mammalian CTCF genes and thus the same DNA-binding potential, but with distinct amino and carboxy termini. We named this gene BORIS for Brother of the Regulator of Imprinted Sites. BORIS is present only in the testis, and expressed in a mutually exclusive manner with CTCF during male germ cell development. We show here that erasure of methylation marks during male germ-line development is associated with dramatic up-regulation of BORIS and down-regulation of CTCF expression. Because BORIS bears the same DNA-binding domain that CTCF employs for recognition of methylation marks in soma, BORIS is a candidate protein for the elusive epigenetic reprogramming factor acting in the male germ line.

Original languageEnglish (US)
Pages (from-to)6806-6811
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number10
DOIs
StatePublished - May 14 2002

ASJC Scopus subject areas

  • General

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