Border cell migration: A model system for live imaging and genetic analysis of collective cell movement

Mohit Prasad, Xiaobo Wang, Li He, Danfeng Cai, Denise J. Montell

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Border cell migration in the Drosophila ovary has emerged as a genetically tractable model for studying collective cell movement. Over many years border cell migration was exclusively studied in fixed samples due to the inability to culture stage 9 egg chambers in vitro. Although culturing late-stage egg chambers was long feasible, stage 9 egg chambers survived only briefly outside the female body. We identified culture conditions that support stage 9 egg chamber development and sustain complete migration of border cells ex vivo. This protocol enables one to compare the dynamics of egg chamber development in wild-type and mutant egg chambers using time-lapse microscopy and taking advantage of a multiposition microscope with a motorized imaging stage. In addition, this protocol has been successfully used in combination with fluorescence resonance energy transfer biosensors, photo-activatable proteins, and pharmacological agents and can be used with wide-field or confocal microscopes in either an upright or an inverted configuration.

Original languageEnglish (US)
Title of host publicationDrosophila Oogenesis: Methods and Protocols
PublisherSpringer New York
Pages89-97
Number of pages9
ISBN (Print)9781493928514, 9781493928507
DOIs
StatePublished - Aug 31 2015

Fingerprint

cell movement
Cell Movement
Ovum
genetic techniques and protocols
Microscopes
Cells
image analysis
Imaging techniques
microscopes
Biosensors
Microscopic examination
biosensors
energy transfer
microscopy
Drosophila
fluorescence
mutants
Fluorescence Resonance Energy Transfer
Proteins
Biosensing Techniques

Keywords

  • Border cell migration
  • Collective cell migration
  • Drosophila stage 9 egg chambers
  • Organ culture
  • Time-lapse live imaging

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Prasad, M., Wang, X., He, L., Cai, D., & Montell, D. J. (2015). Border cell migration: A model system for live imaging and genetic analysis of collective cell movement. In Drosophila Oogenesis: Methods and Protocols (pp. 89-97). Springer New York. https://doi.org/10.1007/978-1-4939-2851-4_6

Border cell migration : A model system for live imaging and genetic analysis of collective cell movement. / Prasad, Mohit; Wang, Xiaobo; He, Li; Cai, Danfeng; Montell, Denise J.

Drosophila Oogenesis: Methods and Protocols. Springer New York, 2015. p. 89-97.

Research output: Chapter in Book/Report/Conference proceedingChapter

Prasad, M, Wang, X, He, L, Cai, D & Montell, DJ 2015, Border cell migration: A model system for live imaging and genetic analysis of collective cell movement. in Drosophila Oogenesis: Methods and Protocols. Springer New York, pp. 89-97. https://doi.org/10.1007/978-1-4939-2851-4_6
Prasad M, Wang X, He L, Cai D, Montell DJ. Border cell migration: A model system for live imaging and genetic analysis of collective cell movement. In Drosophila Oogenesis: Methods and Protocols. Springer New York. 2015. p. 89-97 https://doi.org/10.1007/978-1-4939-2851-4_6
Prasad, Mohit ; Wang, Xiaobo ; He, Li ; Cai, Danfeng ; Montell, Denise J. / Border cell migration : A model system for live imaging and genetic analysis of collective cell movement. Drosophila Oogenesis: Methods and Protocols. Springer New York, 2015. pp. 89-97
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