Boosting with recombinant vaccinia increases HPV-16 E7-specific T cell precursor frequencies of HPV-16 E7-expressing DNA vaccines

Chien Hung Chen; Tian Li Wang; Chien Fu Hung; Drew M. Pardoll; T. C. Wu

doi:10.1016/S0264-410X(99)00528-9

Boosting with recombinant vaccinia increases HPV-16 E7-specific T cell precursor frequencies of HPV-16 E7-expressing DNA vaccines

Chien Hung Chen, Tian Li Wang, Chien Fu Hung, Drew M. Pardoll, T. C. Wu

School of Medicine

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

We have previously linked the sorting signals of the lysosome-associated membrane protein-1 (LAMP-1) to HPV-16 E7 antigen, creating a chimera, Sig/E7/LAMP-1. We found that both Sig/E7/LAMP-1-containing recombinant vaccinia virus (Vac-Sig/E7/LAMP-1) and Sig/E7/LAMP-1 DNA can generate strong antitumor immunity. To determine whether combination of Sig/E7/LAMP-1 DNA and Vac-Sig/E7/LAMP-1 can further enhance immune responses, sequential vaccination with Sig/E7/LAMP-1 DNA and Vac-Sig/E7/LAMP-1 was given. We found that priming with Sig/E7/LAMP-1 DNA and boosting with Vac-Sig/E7/LAMP-1 generated the strongest E7-specific CD8⁺ T cell responses. Our results encourage the use of the DNA prime/vaccinia booster regimen in future clinical trials. Copyright (C) 2000 Elsevier Science Ltd.

Original language	English (US)
Pages (from-to)	2015-2022
Number of pages	8
Journal	Vaccine
Volume	18
Issue number	19
DOIs	https://doi.org/10.1016/S0264-410X(99)00528-9
State	Published - Apr 3 2000

Keywords

DNA vaccine
HPV-16
Vaccinia

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

Access to Document

10.1016/S0264-410X(99)00528-9

Cite this

@article{dd71a2908bb14c8581dc008b1a932be8,

title = "Boosting with recombinant vaccinia increases HPV-16 E7-specific T cell precursor frequencies of HPV-16 E7-expressing DNA vaccines",

abstract = "We have previously linked the sorting signals of the lysosome-associated membrane protein-1 (LAMP-1) to HPV-16 E7 antigen, creating a chimera, Sig/E7/LAMP-1. We found that both Sig/E7/LAMP-1-containing recombinant vaccinia virus (Vac-Sig/E7/LAMP-1) and Sig/E7/LAMP-1 DNA can generate strong antitumor immunity. To determine whether combination of Sig/E7/LAMP-1 DNA and Vac-Sig/E7/LAMP-1 can further enhance immune responses, sequential vaccination with Sig/E7/LAMP-1 DNA and Vac-Sig/E7/LAMP-1 was given. We found that priming with Sig/E7/LAMP-1 DNA and boosting with Vac-Sig/E7/LAMP-1 generated the strongest E7-specific CD8+ T cell responses. Our results encourage the use of the DNA prime/vaccinia booster regimen in future clinical trials. Copyright (C) 2000 Elsevier Science Ltd.",

keywords = "DNA vaccine, HPV-16, Vaccinia",

author = "Chen, {Chien Hung} and Wang, {Tian Li} and Hung, {Chien Fu} and Pardoll, {Drew M.} and Wu, {T. C.}",

note = "Funding Information: We would like to thank Drs Robert J. Kurman and Keetie V. Shah for helpful discussions. We would also like to thank Dr Richard Roden for a critical review of the manuscript and Morris Ling for preparation of the manuscript. This work was supported by NIH 5 po1 34582-01, U19 CA72108-02, RO1 CA72631-01 and the Richard W. TeLinde endowment. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "2000",

month = apr,

day = "3",

doi = "10.1016/S0264-410X(99)00528-9",

language = "English (US)",

volume = "18",

pages = "2015--2022",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

number = "19",

}

TY - JOUR

T1 - Boosting with recombinant vaccinia increases HPV-16 E7-specific T cell precursor frequencies of HPV-16 E7-expressing DNA vaccines

AU - Chen, Chien Hung

AU - Wang, Tian Li

AU - Hung, Chien Fu

AU - Pardoll, Drew M.

AU - Wu, T. C.

N1 - Funding Information: We would like to thank Drs Robert J. Kurman and Keetie V. Shah for helpful discussions. We would also like to thank Dr Richard Roden for a critical review of the manuscript and Morris Ling for preparation of the manuscript. This work was supported by NIH 5 po1 34582-01, U19 CA72108-02, RO1 CA72631-01 and the Richard W. TeLinde endowment. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 2000/4/3

Y1 - 2000/4/3

N2 - We have previously linked the sorting signals of the lysosome-associated membrane protein-1 (LAMP-1) to HPV-16 E7 antigen, creating a chimera, Sig/E7/LAMP-1. We found that both Sig/E7/LAMP-1-containing recombinant vaccinia virus (Vac-Sig/E7/LAMP-1) and Sig/E7/LAMP-1 DNA can generate strong antitumor immunity. To determine whether combination of Sig/E7/LAMP-1 DNA and Vac-Sig/E7/LAMP-1 can further enhance immune responses, sequential vaccination with Sig/E7/LAMP-1 DNA and Vac-Sig/E7/LAMP-1 was given. We found that priming with Sig/E7/LAMP-1 DNA and boosting with Vac-Sig/E7/LAMP-1 generated the strongest E7-specific CD8+ T cell responses. Our results encourage the use of the DNA prime/vaccinia booster regimen in future clinical trials. Copyright (C) 2000 Elsevier Science Ltd.

AB - We have previously linked the sorting signals of the lysosome-associated membrane protein-1 (LAMP-1) to HPV-16 E7 antigen, creating a chimera, Sig/E7/LAMP-1. We found that both Sig/E7/LAMP-1-containing recombinant vaccinia virus (Vac-Sig/E7/LAMP-1) and Sig/E7/LAMP-1 DNA can generate strong antitumor immunity. To determine whether combination of Sig/E7/LAMP-1 DNA and Vac-Sig/E7/LAMP-1 can further enhance immune responses, sequential vaccination with Sig/E7/LAMP-1 DNA and Vac-Sig/E7/LAMP-1 was given. We found that priming with Sig/E7/LAMP-1 DNA and boosting with Vac-Sig/E7/LAMP-1 generated the strongest E7-specific CD8+ T cell responses. Our results encourage the use of the DNA prime/vaccinia booster regimen in future clinical trials. Copyright (C) 2000 Elsevier Science Ltd.

KW - DNA vaccine

KW - HPV-16

KW - Vaccinia

UR - http://www.scopus.com/inward/record.url?scp=0034003977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034003977&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(99)00528-9

DO - 10.1016/S0264-410X(99)00528-9

M3 - Article

C2 - 10706963

AN - SCOPUS:0034003977

SN - 0264-410X

VL - 18

SP - 2015

EP - 2022

JO - Vaccine

JF - Vaccine

IS - 19

ER -

Boosting with recombinant vaccinia increases HPV-16 E7-specific T cell precursor frequencies of HPV-16 E7-expressing DNA vaccines

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this