TY - JOUR
T1 - Bone marrow stem cells assuage radiation-induced damage in a murine model of distraction osteogenesis
T2 - A histomorphometric evaluation
AU - Zheutlin, Alexander R.
AU - Deshpande, Sagar S.
AU - Nelson, Noah S.
AU - Kang, Stephen Y.
AU - Gallagher, Kathleen K.
AU - Polyatskaya, Yekaterina
AU - Rodriguez, Jose J.
AU - Donneys, Alexis
AU - Ranganathan, Kavitha
AU - Buchman, Steven R.
N1 - Publisher Copyright:
© 2016 International Society for Cellular Therapy.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - The purpose of this study is to determine if intraoperatively placed bone marrow stem cells (BMSCs) will permit successful osteocyte and mature bone regeneration in an isogenic murine model of distraction osteogenesis (DO) following radiation therapy (XRT). Lewis rats were split into three groups, DO only (Control), XRT followed by DO (xDO) and XRT followed by DO with intraoperatively placed BMSCs (xDO-BMSC). Coronal sections from the distraction site were obtained, stained and analyzed via statistical analysis with analysis of variance (ANOVA) and subsequent Tukey or Games-Howell post-hoc tests. Comparison of the xDO-BMSC and xDO groups demonstrated significantly improved osteocyte count (87.15 ± 10.19 vs. 67.88 ± 15.38, P = 0.00), and empty lacunae number (2.18 ± 0.79 vs 12.34 ± 6.61, P = 0.00). Quantitative analysis revealed a significant decrease in immature osteoid volume relative to total volume (P = 0.00) and improved the ratio of mature woven bone to immature osteoid (P = 0.02) in the xDO-BMSC compared with the xDO group. No significant differences were found between the Control and xDO-BMSC groups. In an isogenic murine model of DO, BMSC therapy assuaged XRT-induced cellular depletion, resulting in a significant improvement in histological and histomorphometric outcomes.
AB - The purpose of this study is to determine if intraoperatively placed bone marrow stem cells (BMSCs) will permit successful osteocyte and mature bone regeneration in an isogenic murine model of distraction osteogenesis (DO) following radiation therapy (XRT). Lewis rats were split into three groups, DO only (Control), XRT followed by DO (xDO) and XRT followed by DO with intraoperatively placed BMSCs (xDO-BMSC). Coronal sections from the distraction site were obtained, stained and analyzed via statistical analysis with analysis of variance (ANOVA) and subsequent Tukey or Games-Howell post-hoc tests. Comparison of the xDO-BMSC and xDO groups demonstrated significantly improved osteocyte count (87.15 ± 10.19 vs. 67.88 ± 15.38, P = 0.00), and empty lacunae number (2.18 ± 0.79 vs 12.34 ± 6.61, P = 0.00). Quantitative analysis revealed a significant decrease in immature osteoid volume relative to total volume (P = 0.00) and improved the ratio of mature woven bone to immature osteoid (P = 0.02) in the xDO-BMSC compared with the xDO group. No significant differences were found between the Control and xDO-BMSC groups. In an isogenic murine model of DO, BMSC therapy assuaged XRT-induced cellular depletion, resulting in a significant improvement in histological and histomorphometric outcomes.
KW - Histology
KW - Mandible
KW - Osteocyte
KW - Radiotherapy
UR - http://www.scopus.com/inward/record.url?scp=84962045090&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84962045090&partnerID=8YFLogxK
U2 - 10.1016/j.jcyt.2016.01.013
DO - 10.1016/j.jcyt.2016.01.013
M3 - Article
C2 - 27059203
AN - SCOPUS:84962045090
SN - 1465-3249
VL - 18
SP - 664
EP - 672
JO - Cytotherapy
JF - Cytotherapy
IS - 5
ER -