Bone marrow stem cells assuage radiation-induced damage in a murine model of distraction osteogenesis: A histomorphometric evaluation

Alexander R. Zheutlin, Sagar S. Deshpande, Noah S. Nelson, Stephen Y. Kang, Kathleen K. Gallagher, Yekaterina Polyatskaya, Jose J. Rodriguez, Alexis Donneys, Kavitha Ranganathan, Steven R. Buchman

Research output: Contribution to journalArticle

Abstract

The purpose of this study is to determine if intraoperatively placed bone marrow stem cells (BMSCs) will permit successful osteocyte and mature bone regeneration in an isogenic murine model of distraction osteogenesis (DO) following radiation therapy (XRT). Lewis rats were split into three groups, DO only (Control), XRT followed by DO (xDO) and XRT followed by DO with intraoperatively placed BMSCs (xDO-BMSC). Coronal sections from the distraction site were obtained, stained and analyzed via statistical analysis with analysis of variance (ANOVA) and subsequent Tukey or Games-Howell post-hoc tests. Comparison of the xDO-BMSC and xDO groups demonstrated significantly improved osteocyte count (87.15 ± 10.19 vs. 67.88 ± 15.38, P = 0.00), and empty lacunae number (2.18 ± 0.79 vs 12.34 ± 6.61, P = 0.00). Quantitative analysis revealed a significant decrease in immature osteoid volume relative to total volume (P = 0.00) and improved the ratio of mature woven bone to immature osteoid (P = 0.02) in the xDO-BMSC compared with the xDO group. No significant differences were found between the Control and xDO-BMSC groups. In an isogenic murine model of DO, BMSC therapy assuaged XRT-induced cellular depletion, resulting in a significant improvement in histological and histomorphometric outcomes.

Original languageEnglish (US)
Pages (from-to)664-672
Number of pages9
JournalCytotherapy
Volume18
Issue number5
DOIs
StatePublished - May 1 2016
Externally publishedYes

Fingerprint

Distraction Osteogenesis
Bone Marrow Cells
Stem Cells
Radiation
Osteocytes
Bone Regeneration
Cell- and Tissue-Based Therapy
Analysis of Variance
Radiotherapy
Bone and Bones

Keywords

  • Histology
  • Mandible
  • Osteocyte
  • Radiotherapy

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Transplantation
  • Cancer Research

Cite this

Zheutlin, A. R., Deshpande, S. S., Nelson, N. S., Kang, S. Y., Gallagher, K. K., Polyatskaya, Y., ... Buchman, S. R. (2016). Bone marrow stem cells assuage radiation-induced damage in a murine model of distraction osteogenesis: A histomorphometric evaluation. Cytotherapy, 18(5), 664-672. https://doi.org/10.1016/j.jcyt.2016.01.013

Bone marrow stem cells assuage radiation-induced damage in a murine model of distraction osteogenesis : A histomorphometric evaluation. / Zheutlin, Alexander R.; Deshpande, Sagar S.; Nelson, Noah S.; Kang, Stephen Y.; Gallagher, Kathleen K.; Polyatskaya, Yekaterina; Rodriguez, Jose J.; Donneys, Alexis; Ranganathan, Kavitha; Buchman, Steven R.

In: Cytotherapy, Vol. 18, No. 5, 01.05.2016, p. 664-672.

Research output: Contribution to journalArticle

Zheutlin, AR, Deshpande, SS, Nelson, NS, Kang, SY, Gallagher, KK, Polyatskaya, Y, Rodriguez, JJ, Donneys, A, Ranganathan, K & Buchman, SR 2016, 'Bone marrow stem cells assuage radiation-induced damage in a murine model of distraction osteogenesis: A histomorphometric evaluation', Cytotherapy, vol. 18, no. 5, pp. 664-672. https://doi.org/10.1016/j.jcyt.2016.01.013
Zheutlin, Alexander R. ; Deshpande, Sagar S. ; Nelson, Noah S. ; Kang, Stephen Y. ; Gallagher, Kathleen K. ; Polyatskaya, Yekaterina ; Rodriguez, Jose J. ; Donneys, Alexis ; Ranganathan, Kavitha ; Buchman, Steven R. / Bone marrow stem cells assuage radiation-induced damage in a murine model of distraction osteogenesis : A histomorphometric evaluation. In: Cytotherapy. 2016 ; Vol. 18, No. 5. pp. 664-672.
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