Bone marrow cell trafficking following intravenous administration

Jisong Cui, Richard L. Wahl, Tingliang Shen, Susan J. Fisher, Elizabeth Recker, David Ginsburg, Michael W. Long

Research output: Contribution to journalArticlepeer-review

Abstract

To address trafficking of transplanted marrow cells immediately after intravenous infusion, we examined the early fate of infused non-adherent, low-density donor bone marrow cells in a syngeneic mouse model. The presence of infused donor cells, marked with indium-111 oxine (111In), with the fluorescent dye PKH26, or by a detectable transgene marker, was evaluated at 3-48 h in a variety of tissues, including peripheral blood. All three cell- marking methods indicated a rapid (< 4 h) influx of cells into the bone marrow, liver, spleen, muscle and other tissues. Moreover, these tissues remained positive for the 48h observation period. Interestingly, analysis of PKH26-positive cells in non-myeloablated animals demonstrated that approximately 17% of infused donor marrow cells localized to the marrow space within 15h, whereas a smaller proportion of donor cells (~1-2%) localized to the marrow in recipients preconditioned by irradiation. In an effort to enrich for cells that specifically home to the bone marrow, PKH26-labelled donor marrow cells were recovered from the first host and infused into a secondary recipient. Although this was a phenotypically undefined population of cells, no increase was observed in the relative fraction of PKH26-labelled cells returning or 'homing' to the marrow of the second recipient. Taken together, these data suggest both that marrow engraftment may be mediated by non-specific 'seeding' rather than a specific homing signal, and that efficient targeting of transplanted cells to the marrow is a complex multifaceted process.

Original languageEnglish (US)
Pages (from-to)895-902
Number of pages8
JournalBritish journal of haematology
Volume107
Issue number4
DOIs
StatePublished - 1999

Keywords

  • Bone marrow
  • Cell migration
  • Haemopoiesis
  • Homing
  • Transplantation

ASJC Scopus subject areas

  • Hematology

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