Bone geometry and strength measurements in aging mice with the oim mutation

D. J. McBride, Jay Shapiro, M. G. Dunn

Research output: Contribution to journalArticle

Abstract

Mice with the naturally occurring oim mutation allows investigation of bone pathobiology in the setting of one mutation: a G deletion in the murine Cola-2 gene (exon 52) encoding the proα2(I) C-propeptide. As a result, normal sized mRNA is transcribed, but no secreted protein has been identified in oim/oim fibroblasts or osteoblasts. Here we report longitudinal changes in body mass, bone geometry, and bone structural properties of femurs tested in torsion from wild type (+/+) mice and mice homozygous (oim/oim) and heterozygous (+/oim) for the oim mutation. Femurs from mice 3 months, 6 months, 12 months, and >18 months of age were dissected and X-ray films were taken in anterioposterior and mediolateral views to estimate the geometric properties. The metaphyseal ends of femurs were potted in polymethylemethacrylate and mounted on a torsional test fixture designed to convert axial tensile deformation to a torsional load using an INSTRON model 4204 materials tester. Compared with +/+ samples, peak torque at failure was reduced in oim/oim mice. Also, the geometric distribution of midshaft bone for oim/oim mice in terms of cortical area and polar moment was significantly reduced. However, the impact of the mutation on bone distribution was relatively minor for +/oim mice. Consistent with a type III classification in human OI patients, the presence of two nonfunctional alleles in homozygous oim mice significantly reduced body mass compared with age-matched wild type mice. However, no statistical difference in body mass was detected between +/oim and +/+ mice. The absence of a gross phenotypic difference between +/oim and +/+ mice demonstrates a milder phenotype in +/oim mice.

Original languageEnglish (US)
Pages (from-to)172-176
Number of pages5
JournalCalcified Tissue International
Volume62
Issue number2
DOIs
StatePublished - 1998

Fingerprint

Bone and Bones
Mutation
Femur
X-Ray Film
Torque
Osteoblasts
Exons
Fibroblasts
Alleles
Phenotype
Messenger RNA
Genes
Proteins

Keywords

  • Bone
  • Collagen
  • Homotrimer
  • Oim
  • Osteogenesis imperfecta

ASJC Scopus subject areas

  • Endocrinology

Cite this

Bone geometry and strength measurements in aging mice with the oim mutation. / McBride, D. J.; Shapiro, Jay; Dunn, M. G.

In: Calcified Tissue International, Vol. 62, No. 2, 1998, p. 172-176.

Research output: Contribution to journalArticle

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