BMP4 regulates vascular progenitor development in human embryonic stem cells through a Smad-dependent pathway

Hao Bai, Yongxing Gao, Melanie Arzigian, Don M. Wojchowski, Wen Shu Wu, Zack Z. Wang

Research output: Contribution to journalArticle

Abstract

The signals that direct pluripotent stem cell differentiation into lineage-specific cells remain largely unknown. Here, we investigated the roles of BMP on vascular progenitor development from human embryonic stem cells (hESCs). In a serum-free condition, hESCs sequentially differentiated into CD34+CD31-, CD34+CD31+, and then CD34-CD31+ cells during vascular cell development. CD34+CD31+ cells contained vascular progenitor population that gives rise to endothelial cells and smooth muscle cells. BMP4 promoted hESC differentiation into CD34+CD31+ cells at an early stage. In contrast, TGFβ suppressed BMP4-induced CD34+CD31+ cell development, and promoted CD34+CD31- cells that failed to give rise to either endothelial or smooth muscle cells. The BMP-Smad inhibitor, dorsomorphin, inhibited phosphorylation of Smad1/5/8, and blocked hESC differentiation to CD34+CD31+ progenitor cells, suggesting that BMP Smad-dependent signaling is critical for CD34+CD31+ vascular progenitor development. Our findings provide new insight into how pluripotent hESCs differentiate into vascular cells.

Original languageEnglish (US)
Pages (from-to)363-374
Number of pages12
JournalJournal of Cellular Biochemistry
Volume109
Issue number2
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

Fingerprint

Stem cells
Blood Vessels
Cell Differentiation
Muscle
Cells
Smooth Muscle Myocytes
Phosphorylation
Endothelial cells
Pluripotent Stem Cells
Human Embryonic Stem Cells
Stem Cells
Endothelial Cells
Serum
Population

Keywords

  • BMP4
  • Endothelial cells
  • Human embryonic stem cells
  • Smooth muscle cells
  • TGFβ

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

BMP4 regulates vascular progenitor development in human embryonic stem cells through a Smad-dependent pathway. / Bai, Hao; Gao, Yongxing; Arzigian, Melanie; Wojchowski, Don M.; Wu, Wen Shu; Wang, Zack Z.

In: Journal of Cellular Biochemistry, Vol. 109, No. 2, 01.02.2010, p. 363-374.

Research output: Contribution to journalArticle

Bai, Hao ; Gao, Yongxing ; Arzigian, Melanie ; Wojchowski, Don M. ; Wu, Wen Shu ; Wang, Zack Z. / BMP4 regulates vascular progenitor development in human embryonic stem cells through a Smad-dependent pathway. In: Journal of Cellular Biochemistry. 2010 ; Vol. 109, No. 2. pp. 363-374.
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