Abstract
The signals that direct pluripotent stem cell differentiation into lineage-specific cells remain largely unknown. Here, we investigated the roles of BMP on vascular progenitor development from human embryonic stem cells (hESCs). In a serum-free condition, hESCs sequentially differentiated into CD34+CD31-, CD34+CD31+, and then CD34-CD31+ cells during vascular cell development. CD34+CD31+ cells contained vascular progenitor population that gives rise to endothelial cells and smooth muscle cells. BMP4 promoted hESC differentiation into CD34+CD31+ cells at an early stage. In contrast, TGFβ suppressed BMP4-induced CD34+CD31+ cell development, and promoted CD34+CD31- cells that failed to give rise to either endothelial or smooth muscle cells. The BMP-Smad inhibitor, dorsomorphin, inhibited phosphorylation of Smad1/5/8, and blocked hESC differentiation to CD34+CD31+ progenitor cells, suggesting that BMP Smad-dependent signaling is critical for CD34+CD31+ vascular progenitor development. Our findings provide new insight into how pluripotent hESCs differentiate into vascular cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 363-374 |
| Number of pages | 12 |
| Journal | Journal of Cellular Biochemistry |
| Volume | 109 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2010 |
| Externally published | Yes |
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Keywords
- BMP4
- Endothelial cells
- Human embryonic stem cells
- Smooth muscle cells
- TGFβ
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
Cite this
BMP4 regulates vascular progenitor development in human embryonic stem cells through a Smad-dependent pathway. / Bai, Hao; Gao, Yongxing; Arzigian, Melanie; Wojchowski, Don M.; Wu, Wen Shu; Wang, Zack Z.
In: Journal of Cellular Biochemistry, Vol. 109, No. 2, 01.02.2010, p. 363-374.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - BMP4 regulates vascular progenitor development in human embryonic stem cells through a Smad-dependent pathway
AU - Bai, Hao
AU - Gao, Yongxing
AU - Arzigian, Melanie
AU - Wojchowski, Don M.
AU - Wu, Wen Shu
AU - Wang, Zack Z.
PY - 2010/2/1
Y1 - 2010/2/1
N2 - The signals that direct pluripotent stem cell differentiation into lineage-specific cells remain largely unknown. Here, we investigated the roles of BMP on vascular progenitor development from human embryonic stem cells (hESCs). In a serum-free condition, hESCs sequentially differentiated into CD34+CD31-, CD34+CD31+, and then CD34-CD31+ cells during vascular cell development. CD34+CD31+ cells contained vascular progenitor population that gives rise to endothelial cells and smooth muscle cells. BMP4 promoted hESC differentiation into CD34+CD31+ cells at an early stage. In contrast, TGFβ suppressed BMP4-induced CD34+CD31+ cell development, and promoted CD34+CD31- cells that failed to give rise to either endothelial or smooth muscle cells. The BMP-Smad inhibitor, dorsomorphin, inhibited phosphorylation of Smad1/5/8, and blocked hESC differentiation to CD34+CD31+ progenitor cells, suggesting that BMP Smad-dependent signaling is critical for CD34+CD31+ vascular progenitor development. Our findings provide new insight into how pluripotent hESCs differentiate into vascular cells.
AB - The signals that direct pluripotent stem cell differentiation into lineage-specific cells remain largely unknown. Here, we investigated the roles of BMP on vascular progenitor development from human embryonic stem cells (hESCs). In a serum-free condition, hESCs sequentially differentiated into CD34+CD31-, CD34+CD31+, and then CD34-CD31+ cells during vascular cell development. CD34+CD31+ cells contained vascular progenitor population that gives rise to endothelial cells and smooth muscle cells. BMP4 promoted hESC differentiation into CD34+CD31+ cells at an early stage. In contrast, TGFβ suppressed BMP4-induced CD34+CD31+ cell development, and promoted CD34+CD31- cells that failed to give rise to either endothelial or smooth muscle cells. The BMP-Smad inhibitor, dorsomorphin, inhibited phosphorylation of Smad1/5/8, and blocked hESC differentiation to CD34+CD31+ progenitor cells, suggesting that BMP Smad-dependent signaling is critical for CD34+CD31+ vascular progenitor development. Our findings provide new insight into how pluripotent hESCs differentiate into vascular cells.
KW - BMP4
KW - Endothelial cells
KW - Human embryonic stem cells
KW - Smooth muscle cells
KW - TGFβ
UR - http://www.scopus.com/inward/record.url?scp=74949122105&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=74949122105&partnerID=8YFLogxK
U2 - 10.1002/jcb.22410
DO - 10.1002/jcb.22410
M3 - Article
C2 - 19950207
AN - SCOPUS:74949122105
VL - 109
SP - 363
EP - 374
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
SN - 0730-2312
IS - 2
ER -