Blue cones and cone bipolar cells share transcriptional specificity as determined by expression of human blue visual pigment-derived transgenes

M. Isabel Chiu, Jeremy Nathans

Research output: Contribution to journalArticlepeer-review

Abstract

Sequences 5' of the human blue visual pigment gene have been assayed in transgenic mice for their ability to direct cell-type-specific expression of linked β-galactosidase (lacZ) and placental alkaline phosphatase (P ALP 1) reporters. Constructs containing either 5.4 kilobases (kb) or 0.47 kb of 5' flanking DNA direct expression exclusively to the retina. Within the retina, transgene expression is confined to blue cones and cone bipolar cells, as determined, respectively, by double labeling with anti-cone pigment antibodies and by morphologic analyses. These results imply that blue cones and cone bipolar cells have partially overlapping transcriptional specificities.

Original languageEnglish (US)
Pages (from-to)3426-3436
Number of pages11
JournalJournal of Neuroscience
Volume14
Issue number6
DOIs
StatePublished - Jun 1994

Keywords

  • alkaline phosphatase transgenes
  • blue cone visual pigment promoter
  • cone bipolar cells
  • retina-specific expression

ASJC Scopus subject areas

  • Neuroscience(all)

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