Blockade of endogenous B7-H1 suppresses antibacterial protection after primary Listeria monocytogenes infection

Su Kil Seo, Hye Young Jeong, Sae Gwang Park, Soo Woong Lee, Il Whan Choi, Lieping Chen, Inhak Choi

Research output: Contribution to journalArticle

Abstract

B7-H1 (also known as CD274 and PD-L1) is a cosignalling molecule regulating T-cell immunity positively or negatively in vivo. However, little is known about the role of endogenous B7-H1 in bacterial infection. We found that B7-H1 expression was up-regulated in various cell populations including CD4 + and CD8+ T cells, natural killer (NK) cells and macrophages following Listeria monocytogenes infection. Administration of the antagonistic B7-H1 monoclonal antibody resulted in a significant increase in mortality in mice infected with a lethal dose of L. monocytogenes compared with mice given the control immunoglobulin. In vivo blockade of B7-H1 greatly inhibited the production of tumour necrosis factor (TNF)-α and nitric oxide, key effector molecules responsible for intracellular killing by macrophages. B7-H1 blockade also suppressed the expression of granzyme B and interferon (IFN)-γ by NK cells. Interestingly, blocking of endogenous B7-H1 selectively inhibited CD8+ T cells rather than CD4+ T cells in response to L. monocytogenes infection, as evidenced by the reduction of IFN-γ production and the expression of effector surface markers including CD62Lint/low and CD44high in CD8+ T cells from mice given anti-B7-H1 monoclonal antibody. In addition, we found that the proliferation of listeriolysin-O (LLO)-specific and IFN-γ-producing L. monocytogenes-reactive CD8+ T cells was significantly decreased not only in the effector phase but also in the memory phase in the presence of anti-B7-H1 antibody. Our findings thus suggest that endogenous B7-H1 can provide positive costimulatory signals for innate and adaptive immunity leading to protection against intracellular bacterial infection.

Original languageEnglish (US)
Pages (from-to)90-99
Number of pages10
JournalImmunology
Volume123
Issue number1
DOIs
StatePublished - Jan 2008

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Listeriosis
Listeria monocytogenes
T-Lymphocytes
Interferons
Bacterial Infections
Natural Killer Cells
Macrophages
Monoclonal Antibodies
Granzymes
Adaptive Immunity
Innate Immunity
Immunoglobulins
Immunity
Nitric Oxide
Tumor Necrosis Factor-alpha
Mortality
Antibodies
Population

Keywords

  • Adaptive immunity
  • B7-H1
  • Innate immunity
  • Listeria monocytogenes

ASJC Scopus subject areas

  • Immunology

Cite this

Blockade of endogenous B7-H1 suppresses antibacterial protection after primary Listeria monocytogenes infection. / Seo, Su Kil; Jeong, Hye Young; Park, Sae Gwang; Lee, Soo Woong; Choi, Il Whan; Chen, Lieping; Choi, Inhak.

In: Immunology, Vol. 123, No. 1, 01.2008, p. 90-99.

Research output: Contribution to journalArticle

Seo, Su Kil ; Jeong, Hye Young ; Park, Sae Gwang ; Lee, Soo Woong ; Choi, Il Whan ; Chen, Lieping ; Choi, Inhak. / Blockade of endogenous B7-H1 suppresses antibacterial protection after primary Listeria monocytogenes infection. In: Immunology. 2008 ; Vol. 123, No. 1. pp. 90-99.
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AU - Seo, Su Kil

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AB - B7-H1 (also known as CD274 and PD-L1) is a cosignalling molecule regulating T-cell immunity positively or negatively in vivo. However, little is known about the role of endogenous B7-H1 in bacterial infection. We found that B7-H1 expression was up-regulated in various cell populations including CD4 + and CD8+ T cells, natural killer (NK) cells and macrophages following Listeria monocytogenes infection. Administration of the antagonistic B7-H1 monoclonal antibody resulted in a significant increase in mortality in mice infected with a lethal dose of L. monocytogenes compared with mice given the control immunoglobulin. In vivo blockade of B7-H1 greatly inhibited the production of tumour necrosis factor (TNF)-α and nitric oxide, key effector molecules responsible for intracellular killing by macrophages. B7-H1 blockade also suppressed the expression of granzyme B and interferon (IFN)-γ by NK cells. Interestingly, blocking of endogenous B7-H1 selectively inhibited CD8+ T cells rather than CD4+ T cells in response to L. monocytogenes infection, as evidenced by the reduction of IFN-γ production and the expression of effector surface markers including CD62Lint/low and CD44high in CD8+ T cells from mice given anti-B7-H1 monoclonal antibody. In addition, we found that the proliferation of listeriolysin-O (LLO)-specific and IFN-γ-producing L. monocytogenes-reactive CD8+ T cells was significantly decreased not only in the effector phase but also in the memory phase in the presence of anti-B7-H1 antibody. Our findings thus suggest that endogenous B7-H1 can provide positive costimulatory signals for innate and adaptive immunity leading to protection against intracellular bacterial infection.

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