Blastocoel fluid from differentiated blastocysts harbors embryonic genomic material capable of a whole-genome deoxyribonucleic acid amplification and comprehensive chromosome microarray analysis

Kyle J. Tobler, Yulian Zhao, Ric Ross, Andy T. Benner, Xin Xu, Luke Du, Kathleen Broman, Kim Thrift, Paul R. Brezina, William G. Kearns

Research output: Contribution to journalArticlepeer-review

Abstract

Objective To obtain embryonic molecular karyotypes from genomic DNA (deoxyribonucleic acid) isolated from blastocoel fluid (BF) and to compare these karyotypes with the karyotypes from the remaining inner cell mass (ICM) and trophectoderm (TE) of the blastocyst. Design Prospective cohort study. Setting Academic center and preimplantation genetics laboratory. Patient(s) Ninety-six donated cryopreserved embryos. Intervention(s) Embryo biopsy, BF aspiration, DNA analysis using a comparative genomic hybridization microarray (aCGH). Main Outcome Measure(s) The aCGH of a single blastomere, BF-DNA, and ICM-TE. Result(s) The BF-DNA samples resulted in a successful aCGH in 63% of cases. Discordance in karyotypes was found between the BF-DNA and the ICM-TE in 52% of cases. A total of 70% of aneusomic (mosaicism), cleavage-stage embryos differentiated into euploid blastocysts. Probabilities for diagnostic accuracy were calculated and demonstrated the following: sensitivity of 0.88 (95% confidence interval [CI]: 0.62-0.98); specificity of 0.55 (95% CI: 0.39-0.70); positive predictive value of 0.41 (95% CI: 0.25-0.60); negative predictive value of 0.92 (95% CI: 0.75-0.99). Conclusion(s) Genomic DNA from the BF can be amplified and characterized by comprehensive chromosome microarrays. The results demonstrated that aneusomic cleavage-stage embryos differentiated into euploid blastocysts, possibly using a mechanism that marginalizes aneuploid nuclei into the blastocoel cavity. In addition, owing to the high discordance between the karyotypes obtained from the BF-DNA and the ICM-TE, using BF-DNA for preimplantation genetic testing is not yet advised.

Original languageEnglish (US)
Pages (from-to)418-425
Number of pages8
JournalFertility and sterility
Volume104
Issue number2
DOIs
StatePublished - Aug 1 2015

Keywords

  • Blastocoel fluid
  • comparative genomic hybridization
  • microarray
  • preimplantation genetic screening
  • preimplantation genetic testing

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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