Bladder cancer diagnosis during cystoscopy using Raman spectroscopy

M. C M Grimbergen, C. F P Van Swol, R. O P Draga, P. Van Diest, R. M. Verdaasdonk, N. Stone, J. H L R Bosch

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Raman spectroscopy is an optical technique that can be used to obtain specific molecular information of biological tissues. It has been used successfully to differentiate normal and pre-malignant tissue in many organs. The goal of this study is to determine the possibility to distinguish normal tissue from bladder cancer using this system. The endoscopic Raman system consists of a 6 Fr endoscopic probe connected to a 785nm diode laser and a spectral recording system. A total of 107 tissue samples were obtained from 54 patients with known bladder cancer during transurethral tumor resection. Immediately after surgical removal the samples were placed under the Raman probe and spectra were collected and stored for further analysis. The collected spectra were analyzed using multivariate statistical methods. In total 2949 Raman spectra were recorded ex vivo from cold cup biopsy samples with 2 seconds integration time. A multivariate algorithm allowed differentiation of normal and malignant tissue with a sensitivity and specificity of 78,5% and 78,9% respectively. The results show the possibility of discerning normal from malignant bladder tissue by means of Raman spectroscopy using a small fiber based system. Despite the low number of samples the results indicate that it might be possible to use this technique to grade identified bladder wall lesions during endoscopy.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7161
DOIs
StatePublished - 2009
Externally publishedYes
EventPhotonic Therapeutics and Diagnostics V - San Jose, CA, United States
Duration: Jan 24 2009Jan 26 2009

Other

OtherPhotonic Therapeutics and Diagnostics V
CountryUnited States
CitySan Jose, CA
Period1/24/091/26/09

Fingerprint

Bladder Cancer
bladder
Raman Spectroscopy
Raman spectroscopy
cancer
Tissue
Raman
Probe
Endoscopy
Raman Spectra
Biological Tissue
Diode Laser
Time Integration
Differentiate
Statistical method
Specificity
Immediately
Tumor
Biopsy
probes

Keywords

  • Biopsies
  • Bladder cancer
  • Raman spectroscopy

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Grimbergen, M. C. M., Van Swol, C. F. P., Draga, R. O. P., Van Diest, P., Verdaasdonk, R. M., Stone, N., & Bosch, J. H. L. R. (2009). Bladder cancer diagnosis during cystoscopy using Raman spectroscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7161). [716114] https://doi.org/10.1117/12.807811

Bladder cancer diagnosis during cystoscopy using Raman spectroscopy. / Grimbergen, M. C M; Van Swol, C. F P; Draga, R. O P; Van Diest, P.; Verdaasdonk, R. M.; Stone, N.; Bosch, J. H L R.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7161 2009. 716114.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Grimbergen, MCM, Van Swol, CFP, Draga, ROP, Van Diest, P, Verdaasdonk, RM, Stone, N & Bosch, JHLR 2009, Bladder cancer diagnosis during cystoscopy using Raman spectroscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7161, 716114, Photonic Therapeutics and Diagnostics V, San Jose, CA, United States, 1/24/09. https://doi.org/10.1117/12.807811
Grimbergen MCM, Van Swol CFP, Draga ROP, Van Diest P, Verdaasdonk RM, Stone N et al. Bladder cancer diagnosis during cystoscopy using Raman spectroscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7161. 2009. 716114 https://doi.org/10.1117/12.807811
Grimbergen, M. C M ; Van Swol, C. F P ; Draga, R. O P ; Van Diest, P. ; Verdaasdonk, R. M. ; Stone, N. ; Bosch, J. H L R. / Bladder cancer diagnosis during cystoscopy using Raman spectroscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7161 2009.
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