TY - JOUR
T1 - BK polyomavirus reactivation after reduced-intensity double umbilical cord blood cell transplantation
AU - Satyanarayana, Gowri
AU - Hammond, Sarah P.
AU - Broge, Thomas A.
AU - Mackenzie, Matthew R.
AU - Viscidi, Raphael
AU - Politikos, Ioannis
AU - Koralnik, Igor J.
AU - Cutler, Corey S.
AU - Ballen, Karen
AU - Boussiotis, Vassiliki
AU - Marty, Francisco M.
AU - Tan, Chen Sabrina
N1 - Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Serial serum samples from 27 patients who underwent double umbilical cord blood transplantation (dUCBT) were analyzed for BK polyomavirus (BKPyV) DNA by real-time PCR and BKPyV-specific immune globulin by ELISA. Clinical data were collected on all patients. All pre-transplant sera had detectable anti-BKPyV IgG. Fifteen patients (56%) had detectable serum BKPyV DNA (median 8.9×104copies/ml; range 4.1×103-7.9×106copies/ml) a median of 40days (range, 27-733days) after dUCBT, with highest viral loads on Day 100 assessment. The cumulative probability of developing BKPyV viremia by Day 100 was 0.52 (95% CI, 0.33-0.71). Six of 15 patients with BKPyV viremia experienced hemorrhagic cystitis by Day 100. By Day 100, there was a trend towards higher BKPyV viral loads in sera of patients with hemorrhagic cystitis than in those BKPyV viremic patients without hemorrhagic cystitis (p=0.06). BKPyV viremia was associated with significantly higher anti-BKPyV IgM values at 6months post-dUCBT (P=0.003). BKPyV viremia occurs early after dUBCT and is associated with a detectable humoral immune response by 6months post-dUBCT.
AB - Serial serum samples from 27 patients who underwent double umbilical cord blood transplantation (dUCBT) were analyzed for BK polyomavirus (BKPyV) DNA by real-time PCR and BKPyV-specific immune globulin by ELISA. Clinical data were collected on all patients. All pre-transplant sera had detectable anti-BKPyV IgG. Fifteen patients (56%) had detectable serum BKPyV DNA (median 8.9×104copies/ml; range 4.1×103-7.9×106copies/ml) a median of 40days (range, 27-733days) after dUCBT, with highest viral loads on Day 100 assessment. The cumulative probability of developing BKPyV viremia by Day 100 was 0.52 (95% CI, 0.33-0.71). Six of 15 patients with BKPyV viremia experienced hemorrhagic cystitis by Day 100. By Day 100, there was a trend towards higher BKPyV viral loads in sera of patients with hemorrhagic cystitis than in those BKPyV viremic patients without hemorrhagic cystitis (p=0.06). BKPyV viremia was associated with significantly higher anti-BKPyV IgM values at 6months post-dUCBT (P=0.003). BKPyV viremia occurs early after dUBCT and is associated with a detectable humoral immune response by 6months post-dUBCT.
KW - BK polyomavirus
KW - Hemorrhagic cystitis
KW - Viral reactivation
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U2 - 10.1016/j.trim.2014.12.002
DO - 10.1016/j.trim.2014.12.002
M3 - Article
C2 - 25536223
AN - SCOPUS:84926356401
SN - 0966-3274
VL - 32
SP - 116
EP - 120
JO - Transplant Immunology
JF - Transplant Immunology
IS - 2
ER -