Bisphenol A inhibits cultured mouse ovarian follicle growth partially via the aryl hydrocarbon receptor signaling pathway

Ayelet Ziv-Gal, Zelieann R. Craig, Wei Wang, Jodi A. Flaws

Research output: Contribution to journalArticle

Abstract

Bisphenol A (BPA) is an endocrine disruptor that inhibits growth of mouse ovarian follicles and disrupts steroidogenesis at a dose of 438. μM. However, the effects of lower doses of BPA and its mechanism of action in ovarian follicles are unknown. We hypothesized that low doses of BPA inhibit follicular growth and decrease estradiol levels through the aryl hydrocarbon receptor (AHR) pathway. Antral follicles from wild-type and Ahr knock-out (AhrKO) mice were cultured for 96. h. Follicle diameters and estradiol levels then were compared in wild-type and AhrKO follicles ± BPA (0.004-438. μM). BPA inhibited follicle growth (110-438. μM) and decreased estradiol levels (43.8-438. μM) in wild-type and AhrKO follicles. However, at BPA 110. μM, inhibition of growth in AhrKO follicles was attenuated compared to wild-type follicles. These data suggest that BPA may inhibit follicle growth partially via the AHR pathway, whereas its effects on estradiol synthesis likely involve other mechanisms.

Original languageEnglish (US)
Pages (from-to)58-67
Number of pages10
JournalReproductive Toxicology
Volume42
DOIs
StatePublished - Dec 2013
Externally publishedYes

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Keywords

  • AHR
  • Antral follicle
  • BPA
  • Estradiol
  • Low dose
  • Ovary

ASJC Scopus subject areas

  • Toxicology

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