Biphasic Presence of Fibrocytes in a Porcine Hypertrophic Scar Model

Taryn E. Travis, Matthew J. Mino, Lauren T. Moffatt, Neil A. Mauskar, Nicholas J. Prindeze, Pejhman Ghassemi, Jessica C. Ramella-Roman, Marion H. Jordan, Jeffrey W. Shupp

Research output: Contribution to journalArticle

Abstract

The duroc pig has been described as a promising animal model for use in the study of human wound healing and scar formation. However, little is known about the presence and chronology of the fibrocyte cell population in the healing process of these animals. Wounds known to form scar were created on red duroc swine (3" x 3") with a dermatome to a total depth of either 0.06 inches or 0.09 inches. These wounds were allowed to heal completely and biopsies were done at scheduled time points during the healing process. Biopsies were formalin fixed and paraffin embedded for immunohistochemical analysis. Porcine reactive antibodies to CD-45 and procollagen-1 and a human reactive antibody to LSP-1 were used to detect the presence of fibrocytes in immunohistochemistry, an immunocytochemistry. Initial immunohistochemical studies showed evidence of a biphasic presence of fibrocytes. Pigs with 0.06 inches deep wounds showed positive staining for CD-45 and LSP-1 within highly cellular areas at days 2 and 4 after wounding. Additional animals with 0.09 inches deep wounds showed positive staining within similar areas at days 56, 70, and 113 after wounding. There was no immunohistochemical evidence of fibrocytes in skin biopsies taken at days 14, 28, or 42. Procollagen-1 staining was diffused in all samples. Cultured cells were stained for CD-45, LSP-1, and procollagen-1 by immunocytochemistry. These data confirm that fibrocytes are indeed present in this porcine model. We conclude that these cells are present after initial wounding and later during scar formation and remodeling. We believe that this is an evidence of a biphasic presence of fibrocytes, first as an acute response to skin wounding followed by later involvement in the remodeling process, prompted by continued inflammation in a deep partial thickness wound.

Original languageEnglish (US)
Pages (from-to)e125-e135
JournalJournal of Burn Care and Research
Volume36
Issue number3
DOIs
StatePublished - Jul 20 2015
Externally publishedYes

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Hypertrophic Cicatrix
Swine
Procollagen
Wounds and Injuries
Cicatrix
Immunohistochemistry
Staining and Labeling
Biopsy
Chronology
Skin
Antibodies
Paraffin
Wound Healing
Formaldehyde
Cultured Cells
Animal Models
Inflammation
Population

ASJC Scopus subject areas

  • Emergency Medicine
  • Rehabilitation
  • Surgery

Cite this

Travis, T. E., Mino, M. J., Moffatt, L. T., Mauskar, N. A., Prindeze, N. J., Ghassemi, P., ... Shupp, J. W. (2015). Biphasic Presence of Fibrocytes in a Porcine Hypertrophic Scar Model. Journal of Burn Care and Research, 36(3), e125-e135. https://doi.org/10.1097/BCR.0000000000000097

Biphasic Presence of Fibrocytes in a Porcine Hypertrophic Scar Model. / Travis, Taryn E.; Mino, Matthew J.; Moffatt, Lauren T.; Mauskar, Neil A.; Prindeze, Nicholas J.; Ghassemi, Pejhman; Ramella-Roman, Jessica C.; Jordan, Marion H.; Shupp, Jeffrey W.

In: Journal of Burn Care and Research, Vol. 36, No. 3, 20.07.2015, p. e125-e135.

Research output: Contribution to journalArticle

Travis, TE, Mino, MJ, Moffatt, LT, Mauskar, NA, Prindeze, NJ, Ghassemi, P, Ramella-Roman, JC, Jordan, MH & Shupp, JW 2015, 'Biphasic Presence of Fibrocytes in a Porcine Hypertrophic Scar Model', Journal of Burn Care and Research, vol. 36, no. 3, pp. e125-e135. https://doi.org/10.1097/BCR.0000000000000097
Travis TE, Mino MJ, Moffatt LT, Mauskar NA, Prindeze NJ, Ghassemi P et al. Biphasic Presence of Fibrocytes in a Porcine Hypertrophic Scar Model. Journal of Burn Care and Research. 2015 Jul 20;36(3):e125-e135. https://doi.org/10.1097/BCR.0000000000000097
Travis, Taryn E. ; Mino, Matthew J. ; Moffatt, Lauren T. ; Mauskar, Neil A. ; Prindeze, Nicholas J. ; Ghassemi, Pejhman ; Ramella-Roman, Jessica C. ; Jordan, Marion H. ; Shupp, Jeffrey W. / Biphasic Presence of Fibrocytes in a Porcine Hypertrophic Scar Model. In: Journal of Burn Care and Research. 2015 ; Vol. 36, No. 3. pp. e125-e135.
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