Biphasic pattern of histone gene expression during Drosophila oogenesis

A. Ruddell, M. Jacobs-Lorena

Research output: Contribution to journalArticlepeer-review

Abstract

The expression of histone genes during Drosophila oogenesis was compared to periods of DNA synthesis as well as to the pattern of actin gene expression. Accumulation of histone mRNAs was measured by RNA blot hybridization. Relatively low levels of histone mRNAs are present in egg chambers prior to stage 10, during the period of nurse and follicle cell polyploidization. Surprisingly, histone mRNAs accumulate rapidly and selectively after stage 10, coinciding with the onset of nurse cell degeneration and well after DNA synthesis and actin mRNA accumulation have ceased. A large proportion of the histone mRNAs is associated with polysomes at all times, indicating that expression of histone genes is not strictly coupled to DNA synthesis. The burst of histone mRNA accumulation near the end of oogenesis may provide a store of maternal histone mRNA to support the rapid cleavages that occur during early embryogenesis. These and previous results suggest that genes are independently regulated during differentiation of the Drosophila egg chamber.

Original languageEnglish (US)
Pages (from-to)3316-3319
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number10
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Biphasic pattern of histone gene expression during Drosophila oogenesis'. Together they form a unique fingerprint.

Cite this