Biphasic modulation of vascular nitric oxide catabolism by oxygen

Xiaoping Liu, Crystal Cheng, Nicholas Zorko, Scott Cronin, Yeong Renn Chen, Jay L. Zweier

Research output: Contribution to journalArticle

Abstract

Endothelium-derived nitric oxide (NO) plays an important role in the regulation of vascular tone. Lack of NO bioavailability can result in cardiovascular disease. NO bioavailability is determined by its rates of generation and catabolism; however, it is not known how the NO catabolism rate is regulated in the vascular wall under normoxic, hypoxic, and anaerobic conditions. To investigate NO catabolism under different oxygen concentrations, studies of NO and O2 consumption by the isolated rat aorta were performed using electrochemical sensors. Under normoxic conditions, the rate of NO consumption in solution was enhanced in the presence of the rat aorta. Under hypoxic conditions, NO consumption decreased in parallel with the O2 concentration. Like the inhibition of mitochondrial respiration by NO, the inhibitory effects of NO on aortic O2 consumption increased as O 2 concentration decreased. Under anaerobic conditions, however, a paradoxical reacceleration of NO consumption occurred. This increased anaerobic NO consumption was inhibited by the cytochrome c oxidase inhibitor NaCN but not by the free iron chelator deferoxamine, the flavoprotein inhibitor diphenylene iodonium (10 μM), or superoxide dismutase (200 U/ml). The effect of O 2 on the NO consumption could be reproduced by purified cytochrome c oxidase (CcO), implying that CcO is involved in aortic NO catabolism. This reduced NO catabolism at low O2 tensions supports the maintenance of effective NO levels in the vascular wall, reducing the resistance of blood vessels. The increased anaerobic NO catabolism may be important for removing excess NO accumulation in ischemic tissues.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume287
Issue number6 56-6
DOIs
StatePublished - Dec 2004
Externally publishedYes

Fingerprint

Blood Vessels
Nitric Oxide
Oxygen
Electron Transport Complex IV
Biological Availability
Aorta
Flavoproteins
Deferoxamine
Chelating Agents
Superoxide Dismutase
Respiration

Keywords

  • Aorta
  • Cytochrome c oxidase
  • Hypoxia
  • Ischemia

ASJC Scopus subject areas

  • Physiology

Cite this

Biphasic modulation of vascular nitric oxide catabolism by oxygen. / Liu, Xiaoping; Cheng, Crystal; Zorko, Nicholas; Cronin, Scott; Chen, Yeong Renn; Zweier, Jay L.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 287, No. 6 56-6, 12.2004.

Research output: Contribution to journalArticle

Liu, Xiaoping ; Cheng, Crystal ; Zorko, Nicholas ; Cronin, Scott ; Chen, Yeong Renn ; Zweier, Jay L. / Biphasic modulation of vascular nitric oxide catabolism by oxygen. In: American Journal of Physiology - Heart and Circulatory Physiology. 2004 ; Vol. 287, No. 6 56-6.
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