Biosynthesis of titin in cultured skeletal muscle cells

W. B. Isaacs, I. S. Kim, A. Struve, A. B. Fulton

Research output: Contribution to journalArticlepeer-review


Although significant progress has been made regarding the structure and function of titin, little data exist on the biosynthesis of this large protein in developing muscle. Using pulse-labeling with [35S]methionine and immunoprecipitation with an anti-titin mAb, we have examined the biosynthesis of titin in synchronized cultures of skeletal muscle cells derived from day 12 chicken embryos. We find that: (a) titin synthesis increases >4-fold during the first week in culture and during this same period, synthesis of muscle-specific myosin heavy chain increases >12-fold; (b) newly synthesized titin has a t 1/2 of ~70 h; (c) titin is resistant to extraction with Triton X-100 both during and immediately after its synthesis. These observations suggest that newly synthesized titin molecules are stable proteins that rapidly associate with the cytoskeleton of developing myotubes.

Original languageEnglish (US)
Pages (from-to)2189-2195
Number of pages7
JournalJournal of Cell Biology
Issue number5
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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