Biosynthesis of glycosyl phosphatidylinositol protein anchors

Tamara L. Doering; Jayne Raper; Laurence U. Buxbaum; Gerald W. Hart; Paul T. Englund

doi:10.1016/S1046-2023(05)80330-5

Biosynthesis of glycosyl phosphatidylinositol protein anchors

Tamara L. Doering, Jayne Raper, Laurence U. Buxbaum, Gerald W. Hart, Paul T. Englund

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Glycosyl phosphatidylinositols (GPIs) serve as membrane anchors for many plasma membrane proteins in all eukaryotic cells. African trypanosomes provide an ideal system for investigating GPIs, because these parasites produce extremely large quantities to anchor their major surface protein, the variant surface glycoprotein. Through radiolabeling methods and use of a cell-free system, the major features of the trypanosome GPI biosynthetic pathway have been elucidated. It is likely that these techniques, successful in the study of trypanosome GPIs, will be applicable to other eukaryotic cells.

Original language	English (US)
Pages (from-to)	288-296
Number of pages	9
Journal	Methods
Volume	1
Issue number	3
DOIs	https://doi.org/10.1016/S1046-2023(05)80330-5
State	Published - Dec 1990
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology

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title = "Biosynthesis of glycosyl phosphatidylinositol protein anchors",

abstract = "Glycosyl phosphatidylinositols (GPIs) serve as membrane anchors for many plasma membrane proteins in all eukaryotic cells. African trypanosomes provide an ideal system for investigating GPIs, because these parasites produce extremely large quantities to anchor their major surface protein, the variant surface glycoprotein. Through radiolabeling methods and use of a cell-free system, the major features of the trypanosome GPI biosynthetic pathway have been elucidated. It is likely that these techniques, successful in the study of trypanosome GPIs, will be applicable to other eukaryotic cells.",

author = "Doering, {Tamara L.} and Jayne Raper and Buxbaum, {Laurence U.} and Hart, {Gerald W.} and Englund, {Paul T.}",

year = "1990",

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AU - Doering, Tamara L.

AU - Raper, Jayne

AU - Buxbaum, Laurence U.

AU - Hart, Gerald W.

AU - Englund, Paul T.

PY - 1990/12

Y1 - 1990/12

N2 - Glycosyl phosphatidylinositols (GPIs) serve as membrane anchors for many plasma membrane proteins in all eukaryotic cells. African trypanosomes provide an ideal system for investigating GPIs, because these parasites produce extremely large quantities to anchor their major surface protein, the variant surface glycoprotein. Through radiolabeling methods and use of a cell-free system, the major features of the trypanosome GPI biosynthetic pathway have been elucidated. It is likely that these techniques, successful in the study of trypanosome GPIs, will be applicable to other eukaryotic cells.

AB - Glycosyl phosphatidylinositols (GPIs) serve as membrane anchors for many plasma membrane proteins in all eukaryotic cells. African trypanosomes provide an ideal system for investigating GPIs, because these parasites produce extremely large quantities to anchor their major surface protein, the variant surface glycoprotein. Through radiolabeling methods and use of a cell-free system, the major features of the trypanosome GPI biosynthetic pathway have been elucidated. It is likely that these techniques, successful in the study of trypanosome GPIs, will be applicable to other eukaryotic cells.

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VL - 1

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JO - Methods

JF - Methods

IS - 3

ER -

Biosynthesis of glycosyl phosphatidylinositol protein anchors

Abstract

ASJC Scopus subject areas

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