Biosynthesis of glycosaminoglycans by the separated tissues of the embryonic chick cornea

Gerald Warren Hart

Research output: Contribution to journalArticle

Abstract

Corneal tissues (epithelium, endothelium, and stroma) were isolated from chick embryos at 14, 17, and 20 days of incubation and immediately labeled in vitro with d-[6-3H]glucosamine and H2 35SO4. Amount of label incorporated into each type of glycosaminoglycan or into glycopeptides was determined by specific degradative techniques, in conjunction with gel filtration chromatography. Results suggested that corneal epithelium synthesized little, if any, corneal keratan sulfates, but that corneal endothelium may have synthesized small amounts of corneal keratan sulfates. Nearly all corneal keratan sulfates were derived from the stroma. Corneal heparan sulfates appeared to be derived predominantly from corneal epithelium at later stages of development. Corneal endothelium contributed large proportions of the hyaluronic acids of the cornea. Only epithelium produced a large proportion of sulfated glycoproteins. In addition, epithelium synthesized a large proportion of a sulfated, high molecular weight polysaccharide which was resistant to treatments degrading known types of glycosaminoglycans. Each corneal tissue may not only affect corneal morphogenesis directly by contributing a unique spectrum of glycosylated proteins to the extracellular matrix, but also may regulate the extracellular matrix composition indirectly by modulating the biosynthetic activities of the other corneal tissues.

Original languageEnglish (US)
Pages (from-to)78-98
Number of pages21
JournalDevelopmental Biology
Volume62
Issue number1
DOIs
StatePublished - 1978
Externally publishedYes

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Keratan Sulfate
Corneal Epithelium
Glycosaminoglycans
Cornea
Corneal Endothelium
Epithelium
Heparitin Sulfate
Glycopeptides
Extracellular Matrix Proteins
Glucosamine
Chick Embryo
Hyaluronic Acid
Morphogenesis
Endothelium
Gel Chromatography
Extracellular Matrix
Polysaccharides
Glycoproteins
Molecular Weight

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Biosynthesis of glycosaminoglycans by the separated tissues of the embryonic chick cornea. / Hart, Gerald Warren.

In: Developmental Biology, Vol. 62, No. 1, 1978, p. 78-98.

Research output: Contribution to journalArticle

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