Bioreducible poly(Beta-amino ester)s for intracellular delivery of SiRNA

Kristen L. Kozielski, Jordan J. Green

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations


RNA interference (RNAi) is a powerful tool to target and knockdown gene expression in a sequencespecific manner. RNAi can be achieved by the intracellular introduction of SiRNA; however, intracellular SiRNA delivery remains a challenging obstacle. Herein we describe the use of bioreducible nanoparticles formed using poly(beta-amino ester)s (PBAEs) for safe and efficient SiRNA delivery. Methods for polymer synthesis, nanoparticle formation, and SiRNA delivery using these particles are described. A template protocol for nanoparticle screening is presented and can be used to quickly optimize SiRNA delivery for novel applications.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Gene delivery
  • Nanoparticle
  • Polymer
  • RNA interference
  • SiRNA
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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