Bioreducible poly(Beta-amino ester)s for intracellular delivery of SiRNA

Kristen L. Kozielski, Jordan J. Green

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

RNA interference (RNAi) is a powerful tool to target and knockdown gene expression in a sequencespecific manner. RNAi can be achieved by the intracellular introduction of SiRNA; however, intracellular SiRNA delivery remains a challenging obstacle. Herein we describe the use of bioreducible nanoparticles formed using poly(beta-amino ester)s (PBAEs) for safe and efficient SiRNA delivery. Methods for polymer synthesis, nanoparticle formation, and SiRNA delivery using these particles are described. A template protocol for nanoparticle screening is presented and can be used to quickly optimize SiRNA delivery for novel applications.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1364
ISSN (Print)1064-3745

Keywords

  • Gene delivery
  • Nanoparticle
  • Polymer
  • RNA interference
  • SiRNA
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Bioreducible poly(Beta-amino ester)s for intracellular delivery of SiRNA'. Together they form a unique fingerprint.

  • Cite this

    Kozielski, K. L., & Green, J. J. (2016). Bioreducible poly(Beta-amino ester)s for intracellular delivery of SiRNA. In Methods in Molecular Biology (Methods in Molecular Biology; Vol. 1364). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3112-5_8