Bioreactor cultivation of functional bone grafts.

Warren L Grayson, Sarindr Bhumiratana, Christopher Cannizzaro, Gordana Vunjak-Novakovic

Research output: Contribution to journalArticle

Abstract

The clinical demand for functional tissue-engineered bone grafts to regenerate bone defects resulting from trauma and surgical resection of congenital anomalies remains very high. One approach involves the use of human mesenchymal stem cells (hMSCs) that are seeded into biomaterial scaffolds and are induced to generate new bone tissue by osteo-inductive cues. The size of tissue constructs that can be cultured under conventional static conditions is seriously limited by diffusional constraints of nutrient supply resulting from high metabolic activity of bone cells. To cultivate bone constructs of clinically-relevant sizes, it is necessary to utilize perfusion bioreactors, which provides convective transfer of nutrients, and most critically oxygen, to the cells throughout the construct volume. This chapter describes a method for engineering 4-mm thick cylindrical bone grafts using hMSCs (isolated from bone marrow aspirates), biomaterial scaffolds (made of fully decellularized bovine trabecular bone), and a perfusion bioreactor (designed for simultaneous cultivation of six constructs for up to 5 weeks). This approach results in the formation of completely viable, biological bone grafts of clinically relevant sizes.

Original languageEnglish (US)
Pages (from-to)231-241
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume698
DOIs
StatePublished - 2011
Externally publishedYes

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Bioreactors
Transplants
Bone and Bones
Biocompatible Materials
Mesenchymal Stromal Cells
Perfusion
Food
Cues
Bone Marrow
Oxygen
Wounds and Injuries

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Bioreactor cultivation of functional bone grafts. / Grayson, Warren L; Bhumiratana, Sarindr; Cannizzaro, Christopher; Vunjak-Novakovic, Gordana.

In: Methods in molecular biology (Clifton, N.J.), Vol. 698, 2011, p. 231-241.

Research output: Contribution to journalArticle

Grayson, Warren L ; Bhumiratana, Sarindr ; Cannizzaro, Christopher ; Vunjak-Novakovic, Gordana. / Bioreactor cultivation of functional bone grafts. In: Methods in molecular biology (Clifton, N.J.). 2011 ; Vol. 698. pp. 231-241.
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