Biophysics of selectin-mediated cell adhesion

L. S.L. Cheung, P. S. Raman, D. Wirtz, K. Konstantopoulos

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Selectins (L-, P- and E-selectin) are Ca2+-dependent transmembrane glycoproteins presented on the surface of circulating leukocytes, activated platelets, and endothelial cells at sites of inflammation/infection. Selectins bind primarily to sialofucosylated glycoproteins and glycolipids (E-selectin only) on apposing cells, and mediate loose adhesive interactions pertinent to inflammatory disorders and blood-borne metastasis. The fast association and dissociation rates of selectin-ligand bonds coupled to their remarkably high tensile strengths enable them to initiate cell tethering and rolling interactions under physiological flow conditions. This chapter reviews mathematical models and experimental methodologies to highlight the biophysics of selectin-mediated cell adhesion. It summarizes experimental observations on selectin-ligand bond association and dissociation disclosed by different biophysical techniques including micropipette aspiration assays, atomic force microscopy and flow-based adhesion assays. The catch-to-slip selectin-ligand bond dissociation and its contribution to the shear threshold phenomenon are discussed. Understanding the kinetic and micromechanical properties of selectin-ligand bonds mediating cell adhesion pertinent to inflammation and hematogenous metastasis may provide guidelines for developing promising therapies and/or diagnostic tools to combat these disorders.

Original languageEnglish (US)
Title of host publicationComprehensive Biophysics
PublisherElsevier Inc.
Pages10-32
Number of pages23
Volume7
ISBN (Print)9780080957180
DOIs
StatePublished - Dec 1 2012

Keywords

  • Binding kinetics
  • Catch bond
  • Cell adhesion
  • Mathematical models
  • Selectin
  • Shear stress
  • Slip bond

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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