Biophysical cues enhance myogenesis of human adipose derived stem/stromal cells

P. Yilgor Huri, C. A. Cook, D. L. Hutton, B. C. Goh, J. M. Gimble, Douglas DiGirolamo, Warren L Grayson

Research output: Contribution to journalArticle

Abstract

Adipose-derived stem/stromal cell (ASC)-based tissue engineered muscle grafts could provide an effective alternative therapy to autografts - which are limited by their availability - for the regeneration of damaged muscle. However, the current myogenic potential of ASCs is limited by their low differentiation efficiency into myoblasts. The aim of this study was to enhance the myogenic response of human ASCs to biochemical cues by providing biophysical stimuli (11% cyclic uniaxial strain, 0.5. Hz, 1. h/day) to mimic the cues present in the native muscle microenvironment. ASCs elongated and fused upon induction with myogenic induction medium alone. Yet, their myogenic characteristics were significantly enhanced with the addition of biophysical stimulation; the nuclei per cell increased approximately 4.5-fold by day 21 in dynamic compared to static conditions (23.3 ± 7.3 vs. 5.2 ± 1.6, respectively), they aligned at almost 45° to the direction of strain, and exhibited significantly higher expression of myogenic proteins (desmin, myoD and myosin heavy chain). These results demonstrate that mimicking the biophysical cues inherent to the native muscle microenvironment in monolayer ASC cultures significantly improves their differentiation along the myogenic lineage.

Original languageEnglish (US)
Pages (from-to)180-185
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume438
Issue number1
DOIs
StatePublished - Aug 16 2013

Fingerprint

Muscle Development
Stromal Cells
Stem cells
Cues
Muscle
Stem Cells
Muscles
MyoD Protein
Desmin
Myosin Heavy Chains
Myoblasts
Autografts
Complementary Therapies
Cell Nucleus
Cell culture
Grafts
Regeneration
Monolayers
Cell Culture Techniques
Availability

Keywords

  • Adipose-derived stem cell
  • Cyclic strain
  • Dynamic culture
  • Myogenesis
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Biophysical cues enhance myogenesis of human adipose derived stem/stromal cells. / Yilgor Huri, P.; Cook, C. A.; Hutton, D. L.; Goh, B. C.; Gimble, J. M.; DiGirolamo, Douglas; Grayson, Warren L.

In: Biochemical and Biophysical Research Communications, Vol. 438, No. 1, 16.08.2013, p. 180-185.

Research output: Contribution to journalArticle

Yilgor Huri, P. ; Cook, C. A. ; Hutton, D. L. ; Goh, B. C. ; Gimble, J. M. ; DiGirolamo, Douglas ; Grayson, Warren L. / Biophysical cues enhance myogenesis of human adipose derived stem/stromal cells. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 438, No. 1. pp. 180-185.
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