Biomimetic approaches for engineered organ chips and skin electronics for in vitro diagnostics

Kahp Yang Suh, Changhyun Pang, Kyung Jin Jang, Hong Nam Kim, Alex Jiao, Nathaniel S. Hwang, Min Sung Kim, Do Hyun Kang, Deok Ho Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Two kinds of biomimetic systems including engineered organ chip and flexible electronic sensor are presented. First, in vivo, renal tubular epithelial cells are exposed to luminal fluid shear stress (FSS) and a transepithelial osmotic gradient. In this study, we used a simple collecting-duct-on-a-chip to investigate the role of an altered luminal microenvironment in the translocation of aquaporin-2 (AQP2) and the reorganization of actin cytoskeleton (F-actin) in primary cultured inner medullary collecting duct (IMCD) cells of rat kidney. We demonstrate that several factors (i.e., luminal FSS, hormonal stimulation, transepithelial osmotic gradient) collectively exert a profound effect on the AQP2 trafficking in the collecting ducts, which is associated with actin cytoskeletal reorganization. Furthermore, with this kidney-mimicking chip, renal toxicity of cisplatin was tested under static and fluidic conditions, suggesting the physiological relevancy of fluidic environment compared to static culture. Second, we present a simple architecture for a flexible and highly sensitive strain sensor that enables the detection of pressure, shear and torsion. The device is based on two interlocked arrays of high-aspect-ratio Pt-coated polymeric nanofibres that are supported on thin polydimethylsiloxane layers. When different sensing stimuli are applied, the degree of interconnection and the electrical resistance of the sensor changes in a reversible, directional manner with specific, discernible strain-gauge factors. We show that the sensor can be used to monitor signals ranging from human heartbeats to the impact of a bouncing water droplet on a superhydrophobic surface.

Original languageEnglish (US)
Title of host publicationNanosystems in Engineering and Medicine
DOIs
StatePublished - 2012
Externally publishedYes
EventNanosystems in Engineering and Medicine - Incheon, Korea, Republic of
Duration: Sep 10 2012Sep 12 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8548
ISSN (Print)1605-7422

Conference

ConferenceNanosystems in Engineering and Medicine
CountryKorea, Republic of
CityIncheon
Period9/10/129/12/12

Keywords

  • diagnostics
  • in-vitro
  • Organ chips
  • skin electronics

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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