Biological effects of COMT haplotypes and psychosis risk in 22q11.2 deletion syndrome

Doron Gothelf, Amanda J. Law, Amos Frisch, Jingshan Chen, Omer Zarchi, Elena Michaelovsky, Renee Ren-Patterson, Barbara K. Lipska, Miri Carmel, Bhaskar Kolachana, Abraham Weizman, Daniel R. Weinberger

Research output: Contribution to journalArticlepeer-review

Abstract

Background 22q11.2 deletion syndrome (22q11.2DS) is the most common genetic syndrome associated with schizophrenia. The catechol-O-methyltransferase (COMT) gene is located in the obligatory deletion region, and possible associations between COMT variants and neuropsychiatric manifestations in 22q11.2DS have been reported. The purpose of the current study was to evaluate the effect of COMT hemizygosity and molecular haplotypes on gene expression and enzyme activity and its association with psychotic symptoms in 22q11.2DS. Methods Lymphoblast samples were drawn from 53 individuals with 22q11.2DS and 16 typically developing control subjects. We measured COMT messenger (m)RNA and protein expression and enzyme activity using standard procedures. The presence of a psychotic disorder and cognitive deficits were also evaluated using structured testing. Results There was an approximately 50% reduction in COMT mRNA, protein, and enzyme activity levels in 22q11.2DS samples. Haplotype analysis revealed clear phenotypic differences between various Val-containing haplotypes on COMT-3′ untranslated region extended mRNA, soluble COMT and membrane-bound proteins, and enzyme activity. The G variant of rs165599, a 3′ untranslated region single nucleotide polymorphism, was associated with low levels of COMT expression and with the presence of psychosis and lower performance IQ scores in our 22q11.2DS sample. Finally, we demonstrate that the COMT rs74745580 "T" mutation is associated with absent soluble COMT expression and very low COMT activity in two 22q11.2DS individuals. Conclusions Our findings confirm a robust effect of COMT hemizygosity on COMT activity and show complex interactions of variants within the COMT gene that influence COMT biology and confound conclusions based on associations with the Val158Met genotype alone.

Original languageEnglish (US)
Pages (from-to)406-413
Number of pages8
JournalBiological psychiatry
Volume75
Issue number5
DOIs
StatePublished - Mar 1 2014

Keywords

  • COMT
  • DiGeorge
  • gene expression
  • haplotype
  • psychosis
  • velocardiofacial syndrome

ASJC Scopus subject areas

  • Biological Psychiatry

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