Biological characteristics of myelodysplastic syndrome patients who demonstrated high versus no intramedullary apoptosis

Saleem Dar; Suneel Mundle; Tanja Andric; Huma Qawi; Vilasini Shetty; Samina Reza; B. Yifwayimare Mativi; Krishnan Allampallam; Ambereen Ali; Parameswaren Venugopal; Sefer Gezer; La Tanya Broady-Robinson; John Cartlidge; Margaret Showel; Seema Hussaini; Deborah Ragasa; Irfan Ali; Ambreen Chaudhry; Samina Waggoner; Laurie Lisak; Ray Win Huang; Azra Raza

doi:10.1111/j.1600-0609.1999.tb01727.x

Biological characteristics of myelodysplastic syndrome patients who demonstrated high versus no intramedullary apoptosis

Saleem Dar, Suneel Mundle, Tanja Andric, Huma Qawi, Vilasini Shetty, Samina Reza, B. Yifwayimare Mativi, Krishnan Allampallam, Ambereen Ali, Parameswaren Venugopal, Sefer Gezer, La Tanya Broady-Robinson, John Cartlidge, Margaret Showel, Seema Hussaini, Deborah Ragasa, Irfan Ali, Ambreen Chaudhry, Samina Waggoner, Laurie LisakRay Win Huang, Azra Raza

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Spontaneous intramedullary apoptosis was measured in bone marrow (BM) biopsies of 175 patients with myelodysplastic syndromes (MDS) using in situ end-labeling (ISEL) of fragmented DNA. Two groups of high (n = 71) versus low (n = 43) levels of apoptosis were identified while 61 patients were ISEL- negative. Semiquantitative assessment of 3 cytokines, the number of macrophages and in vivo labeling indices (LI) were also determined from consecutive sections of the biopsy. Patients with high apoptosis levels tended to have a high LI (p = 0.013), more macrophages in their BM biopsies (p = 0.006) and higher tumor necrosis factor alpha (TNF-α) levels (not significant) compared to patients with no apoptosis. In addition, low risk MDS patients had significantly lower rates of apoptosis (p = 0.047) and lower levels of TNF-α (p = 0.055) compared to high-risk MDS patients. We conclude that the genesis of cytopenias in MDS is of multifactorial origin and that cytokine-associated apoptosis clearly identifies a distinct biological subgroup of patients who may benefit selectively by use of anti-cytokine therapies.

Original language	English (US)
Pages (from-to)	90-94
Number of pages	5
Journal	European Journal of Haematology
Volume	62
Issue number	2
DOIs	https://doi.org/10.1111/j.1600-0609.1999.tb01727.x
State	Published - 1999
Externally published	Yes

Keywords

Apoptosis
Bone marrow
Human disease
In vivo
Macrophage
Myelodysplastic syndromes (MDS)
Proliferation
Tumor necrosis factor (TNF-α)

ASJC Scopus subject areas

Hematology

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10.1111/j.1600-0609.1999.tb01727.x

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Dar, S., Mundle, S., Andric, T., Qawi, H., Shetty, V., Reza, S., Mativi, B. Y., Allampallam, K., Ali, A., Venugopal, P., Gezer, S., Broady-Robinson, L. T., Cartlidge, J., Showel, M., Hussaini, S., Ragasa, D., Ali, I., Chaudhry, A., Waggoner, S., ... Raza, A. (1999). Biological characteristics of myelodysplastic syndrome patients who demonstrated high versus no intramedullary apoptosis. European Journal of Haematology, 62(2), 90-94. https://doi.org/10.1111/j.1600-0609.1999.tb01727.x

Dar, S, Mundle, S, Andric, T, Qawi, H, Shetty, V, Reza, S, Mativi, BY, Allampallam, K, Ali, A, Venugopal, P, Gezer, S, Broady-Robinson, LT, Cartlidge, J, Showel, M, Hussaini, S, Ragasa, D, Ali, I, Chaudhry, A, Waggoner, S, Lisak, L, Huang, RW & Raza, A 1999, 'Biological characteristics of myelodysplastic syndrome patients who demonstrated high versus no intramedullary apoptosis', European Journal of Haematology, vol. 62, no. 2, pp. 90-94. https://doi.org/10.1111/j.1600-0609.1999.tb01727.x

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title = "Biological characteristics of myelodysplastic syndrome patients who demonstrated high versus no intramedullary apoptosis",

abstract = "Spontaneous intramedullary apoptosis was measured in bone marrow (BM) biopsies of 175 patients with myelodysplastic syndromes (MDS) using in situ end-labeling (ISEL) of fragmented DNA. Two groups of high (n = 71) versus low (n = 43) levels of apoptosis were identified while 61 patients were ISEL- negative. Semiquantitative assessment of 3 cytokines, the number of macrophages and in vivo labeling indices (LI) were also determined from consecutive sections of the biopsy. Patients with high apoptosis levels tended to have a high LI (p = 0.013), more macrophages in their BM biopsies (p = 0.006) and higher tumor necrosis factor alpha (TNF-α) levels (not significant) compared to patients with no apoptosis. In addition, low risk MDS patients had significantly lower rates of apoptosis (p = 0.047) and lower levels of TNF-α (p = 0.055) compared to high-risk MDS patients. We conclude that the genesis of cytopenias in MDS is of multifactorial origin and that cytokine-associated apoptosis clearly identifies a distinct biological subgroup of patients who may benefit selectively by use of anti-cytokine therapies.",

keywords = "Apoptosis, Bone marrow, Human disease, In vivo, Macrophage, Myelodysplastic syndromes (MDS), Proliferation, Tumor necrosis factor (TNF-α)",

author = "Saleem Dar and Suneel Mundle and Tanja Andric and Huma Qawi and Vilasini Shetty and Samina Reza and Mativi, {B. Yifwayimare} and Krishnan Allampallam and Ambereen Ali and Parameswaren Venugopal and Sefer Gezer and Broady-Robinson, {La Tanya} and John Cartlidge and Margaret Showel and Seema Hussaini and Deborah Ragasa and Irfan Ali and Ambreen Chaudhry and Samina Waggoner and Laurie Lisak and Huang, {Ray Win} and Azra Raza",

year = "1999",

doi = "10.1111/j.1600-0609.1999.tb01727.x",

language = "English (US)",

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pages = "90--94",

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AU - Dar, Saleem

AU - Mundle, Suneel

AU - Andric, Tanja

AU - Qawi, Huma

AU - Shetty, Vilasini

AU - Reza, Samina

AU - Mativi, B. Yifwayimare

AU - Allampallam, Krishnan

AU - Ali, Ambereen

AU - Venugopal, Parameswaren

AU - Gezer, Sefer

AU - Broady-Robinson, La Tanya

AU - Cartlidge, John

AU - Showel, Margaret

AU - Hussaini, Seema

AU - Ragasa, Deborah

AU - Ali, Irfan

AU - Chaudhry, Ambreen

AU - Waggoner, Samina

AU - Lisak, Laurie

AU - Huang, Ray Win

AU - Raza, Azra

PY - 1999

Y1 - 1999

N2 - Spontaneous intramedullary apoptosis was measured in bone marrow (BM) biopsies of 175 patients with myelodysplastic syndromes (MDS) using in situ end-labeling (ISEL) of fragmented DNA. Two groups of high (n = 71) versus low (n = 43) levels of apoptosis were identified while 61 patients were ISEL- negative. Semiquantitative assessment of 3 cytokines, the number of macrophages and in vivo labeling indices (LI) were also determined from consecutive sections of the biopsy. Patients with high apoptosis levels tended to have a high LI (p = 0.013), more macrophages in their BM biopsies (p = 0.006) and higher tumor necrosis factor alpha (TNF-α) levels (not significant) compared to patients with no apoptosis. In addition, low risk MDS patients had significantly lower rates of apoptosis (p = 0.047) and lower levels of TNF-α (p = 0.055) compared to high-risk MDS patients. We conclude that the genesis of cytopenias in MDS is of multifactorial origin and that cytokine-associated apoptosis clearly identifies a distinct biological subgroup of patients who may benefit selectively by use of anti-cytokine therapies.

AB - Spontaneous intramedullary apoptosis was measured in bone marrow (BM) biopsies of 175 patients with myelodysplastic syndromes (MDS) using in situ end-labeling (ISEL) of fragmented DNA. Two groups of high (n = 71) versus low (n = 43) levels of apoptosis were identified while 61 patients were ISEL- negative. Semiquantitative assessment of 3 cytokines, the number of macrophages and in vivo labeling indices (LI) were also determined from consecutive sections of the biopsy. Patients with high apoptosis levels tended to have a high LI (p = 0.013), more macrophages in their BM biopsies (p = 0.006) and higher tumor necrosis factor alpha (TNF-α) levels (not significant) compared to patients with no apoptosis. In addition, low risk MDS patients had significantly lower rates of apoptosis (p = 0.047) and lower levels of TNF-α (p = 0.055) compared to high-risk MDS patients. We conclude that the genesis of cytopenias in MDS is of multifactorial origin and that cytokine-associated apoptosis clearly identifies a distinct biological subgroup of patients who may benefit selectively by use of anti-cytokine therapies.

KW - Apoptosis

KW - Bone marrow

KW - Human disease

KW - In vivo

KW - Macrophage

KW - Myelodysplastic syndromes (MDS)

KW - Proliferation

KW - Tumor necrosis factor (TNF-α)

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SN - 0902-4441

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JO - European Journal of Haematology

JF - European Journal of Haematology

IS - 2

ER -

Biological characteristics of myelodysplastic syndrome patients who demonstrated high versus no intramedullary apoptosis

Abstract

Keywords

ASJC Scopus subject areas

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