Biological and biochemical characterization of bovine interleukin 2. Studies with cloned bovine T cells

W. C. Brown, D. J. Grab

Research output: Contribution to journalArticle

Abstract

Bovine peripheral blood lymphocytes stimulated with the T cell mitogen concanavalin A (Con A) secrete a lymphocyte with biological properties similar to T cell growth factor (TCGF) or interleukin 2 (IL 2) from other species. The material supports proliferation of Con A-derived T cell blasts, limiting dilution cloning of T cell blasts, and continuous growth of T cell clones for over 6 mo in vitro. A quantitative microassay with the use of TCGF-dependent, Con A-unresponsive cloned T cells was used to determine the biological activity during purification of IL 2. A single peak of activity with an apparent m.w. of 25,000 to 28,000 was recovered after gel filtration. This material eluted from DEAE-Sephacyl between 135 and 165 mM NaCl. After isoelectric focusing, high pressure liquid chromatography, and gel electrophoresis under reducing conditions, peak IL 2 activity was associated with proteins having m.w. of 20,000 and 23,000.

Original languageEnglish (US)
Pages (from-to)3184-3190
Number of pages7
JournalJournal of Immunology
Volume135
Issue number5
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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