Biological activity of recombinant human interleukin-2 produced in Escherichia coli

Steven A. Rosenberg; Elizabeth A. Grimm; Michael Mcgrogan; Michael Doyle; Ernest Kawasaki; Kirston Koths; David F. Mark

doi:10.1126/science.6367046

Biological activity of recombinant human interleukin-2 produced in Escherichia coli

Steven A. Rosenberg, Elizabeth A. Grimm, Michael Mcgrogan, Michael Doyle, Ernest Kawasaki, Kirston Koths, David F. Mark

Research output: Contribution to journal › Article › peer-review

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Abstract

The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization, No functional differences between native and the recombinant interleukin-2 molecules have been detected.

Original language	English (US)
Pages (from-to)	1412-1415
Number of pages	4
Journal	Science
Volume	223
Issue number	4643
DOIs	https://doi.org/10.1126/science.6367046
State	Published - 1984
Externally published	Yes

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abstract = "The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization, No functional differences between native and the recombinant interleukin-2 molecules have been detected.",

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year = "1984",

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T1 - Biological activity of recombinant human interleukin-2 produced in Escherichia coli

AU - Rosenberg, Steven A.

AU - Grimm, Elizabeth A.

AU - Mcgrogan, Michael

AU - Doyle, Michael

AU - Kawasaki, Ernest

AU - Koths, Kirston

AU - Mark, David F.

PY - 1984

Y1 - 1984

N2 - The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization, No functional differences between native and the recombinant interleukin-2 molecules have been detected.

AB - The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization, No functional differences between native and the recombinant interleukin-2 molecules have been detected.

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Biological activity of recombinant human interleukin-2 produced in Escherichia coli

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