Abstract
The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization, No functional differences between native and the recombinant interleukin-2 molecules have been detected.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1412-1415 |
| Number of pages | 4 |
| Journal | Science |
| Volume | 223 |
| Issue number | 4643 |
| State | Published - Jan 1 1984 |
ASJC Scopus subject areas
- General