Biological activity of recombinant human interleukin-2 produced in Escherichia coli

Steven A. Rosenberg, Elizabeth A. Grimm, Michael Mcgrogan, Michael Doyle, Ernest Kawasaki, Kirston Koths, David F. Mark

Research output: Contribution to journalArticlepeer-review

Abstract

The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization, No functional differences between native and the recombinant interleukin-2 molecules have been detected.

Original languageEnglish (US)
Pages (from-to)1412-1415
Number of pages4
JournalScience
Volume223
Issue number4643
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • General

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