Biological Activities of Aggregated Gamma Globulin I. Skin Reactive and Complement-Fixing Properties of Heat Denatured Gamma Globulin

Teruko Ishizaka; Kimishige Ishizaka

doi:10.3181/00379727-101-25116

Biological Activities of Aggregated Gamma Globulin I. Skin Reactive and Complement-Fixing Properties of Heat Denatured Gamma Globulin

Teruko Ishizaka, Kimishige Ishizaka

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

1. Aggregated human gamma globulin, obtained by heating, gave immediate skin reactions in normal guinea pigs and inactivated complement, whereas aggregated bovine gamma globulin did not have either activity. 2. Aggregated human gamma globulin and antigen-antibody complexes are comparable on weight basis with respect to skin reactivity and complement inactivation. 3. Divalent cation(s) are required for inactivation of complement by aggregated HGG. Inactivation of C'3 by aggregated HGG is markedly dependent upon temperature. 4. A tentative hypothesis concerning the mechanism of complement fixation by antigen-antibody reaction is presented.

Original language	English (US)
Pages (from-to)	845-850
Number of pages	6
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	101
Issue number	4
DOIs	https://doi.org/10.3181/00379727-101-25116
State	Published - Sep 1959

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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abstract = "1. Aggregated human gamma globulin, obtained by heating, gave immediate skin reactions in normal guinea pigs and inactivated complement, whereas aggregated bovine gamma globulin did not have either activity. 2. Aggregated human gamma globulin and antigen-antibody complexes are comparable on weight basis with respect to skin reactivity and complement inactivation. 3. Divalent cation(s) are required for inactivation of complement by aggregated HGG. Inactivation of C'3 by aggregated HGG is markedly dependent upon temperature. 4. A tentative hypothesis concerning the mechanism of complement fixation by antigen-antibody reaction is presented.",

author = "Teruko Ishizaka and Kimishige Ishizaka",

year = "1959",

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AU - Ishizaka, Teruko

AU - Ishizaka, Kimishige

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N2 - 1. Aggregated human gamma globulin, obtained by heating, gave immediate skin reactions in normal guinea pigs and inactivated complement, whereas aggregated bovine gamma globulin did not have either activity. 2. Aggregated human gamma globulin and antigen-antibody complexes are comparable on weight basis with respect to skin reactivity and complement inactivation. 3. Divalent cation(s) are required for inactivation of complement by aggregated HGG. Inactivation of C'3 by aggregated HGG is markedly dependent upon temperature. 4. A tentative hypothesis concerning the mechanism of complement fixation by antigen-antibody reaction is presented.

AB - 1. Aggregated human gamma globulin, obtained by heating, gave immediate skin reactions in normal guinea pigs and inactivated complement, whereas aggregated bovine gamma globulin did not have either activity. 2. Aggregated human gamma globulin and antigen-antibody complexes are comparable on weight basis with respect to skin reactivity and complement inactivation. 3. Divalent cation(s) are required for inactivation of complement by aggregated HGG. Inactivation of C'3 by aggregated HGG is markedly dependent upon temperature. 4. A tentative hypothesis concerning the mechanism of complement fixation by antigen-antibody reaction is presented.

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