TY - JOUR
T1 - Biological Activities of Aggregated Gamma Globulin I. Skin Reactive and Complement-Fixing Properties of Heat Denatured Gamma Globulin
AU - Ishizaka, Teruko
AU - Ishizaka, Kimishige
N1 - Funding Information:
These experiments were performed while the authors were in residence in Depts. of Microbiology and Medicine, Johns Hopkins School of Medicine and Public Health. This work was done under auspices of Commission on Cutaneous Diseases of Armed Forces Epidemiological Board, and supported by Office of Surgeon General, Department of Army, and National Science Fn.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1959/9
Y1 - 1959/9
N2 - 1. Aggregated human gamma globulin, obtained by heating, gave immediate skin reactions in normal guinea pigs and inactivated complement, whereas aggregated bovine gamma globulin did not have either activity. 2. Aggregated human gamma globulin and antigen-antibody complexes are comparable on weight basis with respect to skin reactivity and complement inactivation. 3. Divalent cation(s) are required for inactivation of complement by aggregated HGG. Inactivation of C'3 by aggregated HGG is markedly dependent upon temperature. 4. A tentative hypothesis concerning the mechanism of complement fixation by antigen-antibody reaction is presented.
AB - 1. Aggregated human gamma globulin, obtained by heating, gave immediate skin reactions in normal guinea pigs and inactivated complement, whereas aggregated bovine gamma globulin did not have either activity. 2. Aggregated human gamma globulin and antigen-antibody complexes are comparable on weight basis with respect to skin reactivity and complement inactivation. 3. Divalent cation(s) are required for inactivation of complement by aggregated HGG. Inactivation of C'3 by aggregated HGG is markedly dependent upon temperature. 4. A tentative hypothesis concerning the mechanism of complement fixation by antigen-antibody reaction is presented.
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U2 - 10.3181/00379727-101-25116
DO - 10.3181/00379727-101-25116
M3 - Article
C2 - 14405924
AN - SCOPUS:70449303089
VL - 101
SP - 845
EP - 850
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
SN - 0037-9727
IS - 4
ER -