Biologic scaffold composed of skeletal muscle extracellular matrix

Matthew T. Wolf, Kerry A. Daly, Janet E. Reing, Stephen F. Badylak

Research output: Contribution to journalArticle

Abstract

Biologic scaffolds prepared from the extracellular matrix (ECM) of decellularized mammalian tissues have been shown to facilitate constructive remodeling in injured tissues such as skeletal muscle, the esophagus, and lower urinary tract, among others. The ECM of every tissue has a unique composition and structure that likely has direct effects on the host response and it is plausible that ECM harvested from a given tissue would provide distinct advantages over ECM harvested from nonhomologous tissues. For example, a tissue specific muscle ECM scaffold may be more suitable for constructive remodeling of skeletal muscle than non-homologous ECM tissue sources. The present study describes an enzymatic and chemical decellularization process for isolating skeletal muscle ECM scaffolds using established decellularization criteria and characterized the structure and chemical composition of the resulting ECM. The results were compared to those from a non-muscle ECM derived from small intestine (SIS). Muscle ECM was shown to contain growth factors, glycosaminoglycans, and basement membrane structural proteins which differed from those present in SIS. Myogenic cells survived and proliferated on muscle ECM scaffolds invitro, and when implanted in a rat abdominal wall injury model invivo was shown to induce a constructive remodeling response associated with scaffold degradation and myogenesis in the implant area; however, the remodeling outcome did not differ from that induced by SIS by 35 days post surgery. These results suggest that superior tissue remodeling outcomes are not universally dependent upon homologous tissue derived ECM scaffold materials.

Original languageEnglish (US)
Pages (from-to)2916-2925
Number of pages10
JournalBiomaterials
Volume33
Issue number10
DOIs
StatePublished - Apr 1 2012
Externally publishedYes

Fingerprint

Scaffolds
Extracellular Matrix
Muscle
Skeletal Muscle
Tissue
Small Intestine
Muscles
Chemical Phenomena
Glycosaminoglycans
Chemical analysis
Abdominal Injuries
Surgery
Muscle Development
Rats
Intercellular Signaling Peptides and Proteins
Abdominal Wall
Urinary Tract
Ambulatory Surgical Procedures
Basement Membrane
Esophagus

Keywords

  • Animal model
  • ECM (extracellular matrix)
  • Muscle
  • Regenerative medicine
  • Scaffold

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Biologic scaffold composed of skeletal muscle extracellular matrix. / Wolf, Matthew T.; Daly, Kerry A.; Reing, Janet E.; Badylak, Stephen F.

In: Biomaterials, Vol. 33, No. 10, 01.04.2012, p. 2916-2925.

Research output: Contribution to journalArticle

Wolf, Matthew T. ; Daly, Kerry A. ; Reing, Janet E. ; Badylak, Stephen F. / Biologic scaffold composed of skeletal muscle extracellular matrix. In: Biomaterials. 2012 ; Vol. 33, No. 10. pp. 2916-2925.
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