Biologic activities of soluble antigen-antibody complexes. X. Skin-reactive properties of allergen-reagin complexes

Kimishige Ishizaka; Teruko Ishizaka

doi:10.1016/0021-8707(65)90034-1

Biologic activities of soluble antigen-antibody complexes. X. Skin-reactive properties of allergen-reagin complexes

Kimishige Ishizaka, Teruko Ishizaka

School of Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Mixtures of ragweed pollen extract with a fraction of an allergic serum containing reagin gave erythema-wheal reactions in normal individuals. Skin reactivity was not induced either with the fraction which had been heated at 56° C. for 4 hours or with the γ₂-globulin fraction of the same serum. The skin reactions with the allergen-reagin mixtures were inhibited by prior treatment of the skin sites with normal γ_1A-globulin, but not with γ₂- or γ_1M-globulin. After removal of free allergen in the mixture by gel filtration, no reaginic activity was recovered. A skin-reactive substance was eluted from a Sephadex G-200 column in the fractions containing γ_1A-globulin. The results definitely indicate that allergen-reagin complexes formed in vitro have an activity to induce erythema-wheal reactions in normal individuals.

Original language	English (US)
Pages (from-to)	70-83
Number of pages	14
Journal	Journal of Allergy
Volume	36
Issue number	1
DOIs	https://doi.org/10.1016/0021-8707(65)90034-1
State	Published - 1965

ASJC Scopus subject areas

General Medicine

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10.1016/0021-8707(65)90034-1

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title = "Biologic activities of soluble antigen-antibody complexes. X. Skin-reactive properties of allergen-reagin complexes",

abstract = "Mixtures of ragweed pollen extract with a fraction of an allergic serum containing reagin gave erythema-wheal reactions in normal individuals. Skin reactivity was not induced either with the fraction which had been heated at 56° C. for 4 hours or with the γ2-globulin fraction of the same serum. The skin reactions with the allergen-reagin mixtures were inhibited by prior treatment of the skin sites with normal γ1A-globulin, but not with γ2- or γ1M-globulin. After removal of free allergen in the mixture by gel filtration, no reaginic activity was recovered. A skin-reactive substance was eluted from a Sephadex G-200 column in the fractions containing γ1A-globulin. The results definitely indicate that allergen-reagin complexes formed in vitro have an activity to induce erythema-wheal reactions in normal individuals.",

author = "Kimishige Ishizaka and Teruko Ishizaka",

note = "Funding Information: From the Children{\textquoteright}s Asthma This work was supported Health Service. The results of investigation American Association of Immunologists, Received for publication May 19, Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "1965",

doi = "10.1016/0021-8707(65)90034-1",

language = "English (US)",

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journal = "Journal of Allergy",

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T1 - Biologic activities of soluble antigen-antibody complexes. X. Skin-reactive properties of allergen-reagin complexes

AU - Ishizaka, Kimishige

AU - Ishizaka, Teruko

N1 - Funding Information: From the Children’s Asthma This work was supported Health Service. The results of investigation American Association of Immunologists, Received for publication May 19, Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1965

Y1 - 1965

N2 - Mixtures of ragweed pollen extract with a fraction of an allergic serum containing reagin gave erythema-wheal reactions in normal individuals. Skin reactivity was not induced either with the fraction which had been heated at 56° C. for 4 hours or with the γ2-globulin fraction of the same serum. The skin reactions with the allergen-reagin mixtures were inhibited by prior treatment of the skin sites with normal γ1A-globulin, but not with γ2- or γ1M-globulin. After removal of free allergen in the mixture by gel filtration, no reaginic activity was recovered. A skin-reactive substance was eluted from a Sephadex G-200 column in the fractions containing γ1A-globulin. The results definitely indicate that allergen-reagin complexes formed in vitro have an activity to induce erythema-wheal reactions in normal individuals.

AB - Mixtures of ragweed pollen extract with a fraction of an allergic serum containing reagin gave erythema-wheal reactions in normal individuals. Skin reactivity was not induced either with the fraction which had been heated at 56° C. for 4 hours or with the γ2-globulin fraction of the same serum. The skin reactions with the allergen-reagin mixtures were inhibited by prior treatment of the skin sites with normal γ1A-globulin, but not with γ2- or γ1M-globulin. After removal of free allergen in the mixture by gel filtration, no reaginic activity was recovered. A skin-reactive substance was eluted from a Sephadex G-200 column in the fractions containing γ1A-globulin. The results definitely indicate that allergen-reagin complexes formed in vitro have an activity to induce erythema-wheal reactions in normal individuals.

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Biologic activities of soluble antigen-antibody complexes. X. Skin-reactive properties of allergen-reagin complexes

Abstract

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