Bioinformatics and mass spectrometry for microorganism identification: Proteome-wide post-translational modifications and database search algorithms for characterization of intact H. pylori

P. A. Demirev, J. S. Lin, Fernando J Pineda, C. Fenselau

Research output: Contribution to journalArticle

Abstract

MALDI-TOF mass spectrometry has been coupled with Internet-based proteome database search algorithms in an approach for direct microorganism identification. This approach is applied here to characterize intact H. pylori (strain 26695) Gram-negative bacteria, the most ubiquitous human pathogen. A procedure for including a specific and common posttranslational modification, N-terminal Met cleavage, in the search algorithm is described. Accounting for posttranslational modifications in putative protein biomarkers improves the identification reliability by at least an order of magnitude. The influence of other factors, such as number of detected biomarker peaks, proteome size, spectral calibration, and mass accuracy, on the microorganism identification success rate is illustrated as well.

Original languageEnglish (US)
Pages (from-to)4566-4573
Number of pages8
JournalAnalytical Chemistry
Volume73
Issue number19
DOIs
Publication statusPublished - Oct 1 2001

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ASJC Scopus subject areas

  • Analytical Chemistry

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