@article{e1735a5f88fc4d959ffb3993138a8083,
title = "Biofilm-inspired encapsulation of probiotics for the treatment of complex infections",
abstract = "The emergence of antimicrobial resistance poses a major challenge to healthcare. Probiotics offer a potential alternative treatment method but are often incompatible with antibiotics themselves, diminishing their overall therapeutic utility. This work uses biofilm-inspired encapsulation of probiotics to confer temporary antibiotic protection and to enable the coadministration of probiotics and antibiotics. Probiotics are encapsulated within alginate, a crucial component of pseudomonas biofilms, based on a simple two-step alginate cross-linking procedure. Following exposure to the antibiotic tobramycin, the growth and metabolic activity of encapsulated probiotics are unaffected by tobramycin, and they show a four-log survival advantage over free probiotics. This results from tobramycin sequestration on the periphery of alginate beads which prevents its diffusion into the core but yet allows probiotic byproducts to diffuse outward. It is demonstrated that this approach using tobramycin combined with encapsulated probiotic has the ability to completely eradicate methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa in coculture, the two most widely implicated bacteria in chronic wounds.",
keywords = "Alginate, Antimicrobial resistance, Chronic wounds, Encapsulation, Probiotics",
author = "Zhihao Li and Behrens, {Adam M.} and Nitzan Ginat and Tzeng, {Stephany Y.} and Xueguang Lu and Sarit Sivan and Robert Langer and Ana Jaklenec",
note = "Funding Information: Z.L. was supported by the Janggen-P{\"o}hn-Foundation and by Beatrice Beck-Schimmer and Hans-Ruedi Gonzenbach. The authors would also like to thank Wen Tang for her assistance with fluorescence-{\L}labeling of alginate and Patrick Boisvert from the MIT CMSE facility for his assistance with electron microscopy. The authors thank the Koch Institute Swanson Biotechnology Center for technical support, specifically Eliza Vasile from the Microscopy core facility for her assistance with confocal microscopy and Richard Cook from the Biopolymers & Proteomics Core Facility for his assistance with HPLC and MALDI. Note: The last sentence in the first paragraph on page 4 [“The protection of encapsulated Bio-K+ against tobramycin is therefore likely due to the electrostatic interaction of cationic tobramycin and polyanionic alginate.”] was corrected on December 17, 2018, after inititial publication online. Funding Information: Z.L. was supported by the Janggen-P?hn-Foundation and by Beatrice Beck-Schimmer and Hans-Ruedi Gonzenbach. The authors would also like to thank Wen Tang for her assistance with fluorescence-?labeling of alginate and Patrick Boisvert from the MIT CMSE facility for his assistance with electron microscopy. The authors thank the Koch Institute Swanson Biotechnology Center for technical support, specifically Eliza Vasile from the Microscopy core facility for her assistance with confocal microscopy and Richard Cook from the Biopolymers & Proteomics Core Facility for his assistance with HPLC and MALDI. Note: The last sentence in the first paragraph on page 4 [?The protection of encapsulated Bio-K+ against tobramycin is therefore likely due to the electrostatic interaction of cationic tobramycin and polyanionic alginate.?] was corrected on December 17, 2018, after inititial publication online. Publisher Copyright: {\textcopyright} 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2018",
month = dec,
doi = "10.1002/adma.201803925",
language = "English (US)",
volume = "30",
journal = "Advanced Materials",
issn = "0935-9648",
publisher = "Wiley-VCH Verlag",
number = "51",
}