TY - JOUR
T1 - Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica)
AU - Michaud, Luigi
AU - Di Cello, Francescopaolo
AU - Brilli, Matteo
AU - Fani, Renato
AU - Lo Giudice, Angelina
AU - Bruni, Vivia
PY - 2004/1/15
Y1 - 2004/1/15
N2 - A set of 146 Antarctic marine isolates from the Ross Sea was characterized by a combination of molecular techniques in order to determine the degree of inter- and intraspecific variability. Isolates were analyzed by amplified rDNA restriction analysis (ARDRA) using the tetrameric enzyme AluI, resulting in 52 different groups, corresponding to at least 52 different bacterial species, indicating a high degree of interspecific variability. The phylogenetic position of bacteria belonging to some ARDRA groups was obtained by sequencing of 16S rDNA. Random amplified polymorphic DNA (RAPD) analysis, carried out on the largest ARDRA groups, revealed a high intraspecific genetic variability, too. The analysis of plasmid content revealed the existence of horizontal gene transfer between strains belonging to the same and to different species. A comparison of the whole body of morphological, physiological and biochemical data was finally carried out.
AB - A set of 146 Antarctic marine isolates from the Ross Sea was characterized by a combination of molecular techniques in order to determine the degree of inter- and intraspecific variability. Isolates were analyzed by amplified rDNA restriction analysis (ARDRA) using the tetrameric enzyme AluI, resulting in 52 different groups, corresponding to at least 52 different bacterial species, indicating a high degree of interspecific variability. The phylogenetic position of bacteria belonging to some ARDRA groups was obtained by sequencing of 16S rDNA. Random amplified polymorphic DNA (RAPD) analysis, carried out on the largest ARDRA groups, revealed a high intraspecific genetic variability, too. The analysis of plasmid content revealed the existence of horizontal gene transfer between strains belonging to the same and to different species. A comparison of the whole body of morphological, physiological and biochemical data was finally carried out.
KW - 16S rDNA
KW - Amplified rDNA restriction analysis
KW - Antarctic marine bacteria
KW - Biodiversity
KW - Random amplified polymorphic DNA analysis
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U2 - 10.1016/S0378-1097(03)00857-7
DO - 10.1016/S0378-1097(03)00857-7
M3 - Article
C2 - 14734167
AN - SCOPUS:0347753489
SN - 0378-1097
VL - 230
SP - 63
EP - 71
JO - FEMS microbiology letters
JF - FEMS microbiology letters
IS - 1
ER -