Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica)

Luigi Michaud, Francescopaolo Di Cello, Matteo Brilli, Renato Fani, Angelina Lo Giudice, Vivia Bruni

Research output: Contribution to journalArticlepeer-review

Abstract

A set of 146 Antarctic marine isolates from the Ross Sea was characterized by a combination of molecular techniques in order to determine the degree of inter- and intraspecific variability. Isolates were analyzed by amplified rDNA restriction analysis (ARDRA) using the tetrameric enzyme AluI, resulting in 52 different groups, corresponding to at least 52 different bacterial species, indicating a high degree of interspecific variability. The phylogenetic position of bacteria belonging to some ARDRA groups was obtained by sequencing of 16S rDNA. Random amplified polymorphic DNA (RAPD) analysis, carried out on the largest ARDRA groups, revealed a high intraspecific genetic variability, too. The analysis of plasmid content revealed the existence of horizontal gene transfer between strains belonging to the same and to different species. A comparison of the whole body of morphological, physiological and biochemical data was finally carried out.

Original languageEnglish (US)
Pages (from-to)63-71
Number of pages9
JournalFEMS microbiology letters
Volume230
Issue number1
DOIs
StatePublished - Jan 15 2004

Keywords

  • 16S rDNA
  • Amplified rDNA restriction analysis
  • Antarctic marine bacteria
  • Biodiversity
  • Random amplified polymorphic DNA analysis

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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