Bioconversion of novel substrate Saccharum spontaneum, a weedy material, into ethanol by Pichia stipitis NCIM3498

Anuj K. Chandel, Om V. Singh, L. Venkateswar Rao, G. Chandrasekhar, M. Lakshmi Narasu

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Rising fuel prices and environmental issues have paved the way for the exploration of cellulosic ethanol. However, challenges involving substrate hydrolysis and cost-effectiveness still limit the efficient bioconversion and utilization of cellulosic ethanol. We aimed to evaluate a cheaper and abundantly available wild sugarcane variety, Saccharum spontaneum, as the raw substrate for bioconversion of ethanol by Pichia stipitis NCIM3498. Three different strategies for substrate hydrolysis using acid (dilute sulfuric acid) and alkali (dilute sodium hydroxide) and aqueous ammonia (AA) treatment followed by enzymatic hydrolysis were studied. A maximum of 631.5 ± 3.25. mg/g sugars with 89.38% hydrolytic efficiency (HE) could be achieved after enzymatic hydrolysis of AA-pretreated S. spontaneum. All the substrate hydrolysates were evaluated for ethanol conversion in batches by P. stipitis. The microbial fermentation of released sugars into ethanol showed (g/g) 0.36 ± 0.011, 0.384 ± 0.022, 0.391 ± 0.02, and 0.40 ± 0.01 yield from detoxified acid hydrolysate and acid-, NaOH- and AA-pretreated substrate S. spontaneum enzymatic hydrolysates, respectively.

Original languageEnglish (US)
Pages (from-to)1709-1714
Number of pages6
JournalBioresource Technology
Issue number2
StatePublished - Jan 2011
Externally publishedYes


  • Bioethanol
  • Enzymatic hydrolysis
  • Fermentation
  • Pichia stipitis NCIM 3498
  • Saccharum spontaneum

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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