TY - JOUR
T1 - Biocompatible Conjugated Polymer Nanoparticles for Efficient Photothermal Tumor Therapy
AU - Geng, Junlong
AU - Sun, Chunyang
AU - Liu, Jie
AU - Liao, Lun De
AU - Yuan, Youyong
AU - Thakor, Nitish
AU - Wang, Jun
AU - Liu, Bin
N1 - Funding Information:
J. L. Geng and C. Y. Sun contributed equally to this work. We are grateful to the financial support from the Singapore National Research Foundation (R‐279–000–323–281), the JCO IMRE/12–8P1103 and JCO IMRE/14‐8P1110. Mr. J. L. Geng thanks the National University of Singapore for the research scholarship.
Publisher Copyright:
© 2014 Wiley-VCH Verlag GmbH & Co. KGaA.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Conjugated polymers (CPs) with strong near-infrared (NIR) absorption and high heat conversion efficiency have emerged as a new generation of photothermal therapy (PTT) agents for cancer therapy. An efficient strategy to design NIR absorbing CPs with good water dispersibility is essential to achieve excellent therapeutic effect. In this work, poly[9,9-bis(4-(2-ethylhexyl)phenyl)fluorene-alt-co-6,7-bis(4-(hexyloxy)phenyl)-4,9-di(thiophen-2-yl)-thiadiazoloquinoxaline] (PFTTQ) is synthesized through the combination of donor-acceptor moieties by Suzuki polymerization. PFTTQ nanoparticles (NPs) are fabricated through a precipitation approach using 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) as the encapsulation matrix. Due to the large NIR absorption coefficient (3.6 L g-1 cm-1), the temperature of PFTTQ NP suspension (0.5 mg/mL) could be rapidly increased to more than 50 °C upon continuous 808 nm laser irradiation (0.75 W/cm2) for 5 min. The PFTTQ NPs show good biocompatibility to both MDA-MB-231 cells and Hela cells at 400 μg/mL of NPs, while upon laser irradiation, effective cancer cell killing is observed at a NP concentration of 50 μg/mL. Moreover, PFTTQ NPs could efficiently ablate tumor in in vivo study using a Hela tumor mouse model. Considering the large amount of NIR absorbing CPs available, the general encapsulation strategy will enable the development of more efficient PTT agents for cancer or tumor therapy.
AB - Conjugated polymers (CPs) with strong near-infrared (NIR) absorption and high heat conversion efficiency have emerged as a new generation of photothermal therapy (PTT) agents for cancer therapy. An efficient strategy to design NIR absorbing CPs with good water dispersibility is essential to achieve excellent therapeutic effect. In this work, poly[9,9-bis(4-(2-ethylhexyl)phenyl)fluorene-alt-co-6,7-bis(4-(hexyloxy)phenyl)-4,9-di(thiophen-2-yl)-thiadiazoloquinoxaline] (PFTTQ) is synthesized through the combination of donor-acceptor moieties by Suzuki polymerization. PFTTQ nanoparticles (NPs) are fabricated through a precipitation approach using 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) as the encapsulation matrix. Due to the large NIR absorption coefficient (3.6 L g-1 cm-1), the temperature of PFTTQ NP suspension (0.5 mg/mL) could be rapidly increased to more than 50 °C upon continuous 808 nm laser irradiation (0.75 W/cm2) for 5 min. The PFTTQ NPs show good biocompatibility to both MDA-MB-231 cells and Hela cells at 400 μg/mL of NPs, while upon laser irradiation, effective cancer cell killing is observed at a NP concentration of 50 μg/mL. Moreover, PFTTQ NPs could efficiently ablate tumor in in vivo study using a Hela tumor mouse model. Considering the large amount of NIR absorbing CPs available, the general encapsulation strategy will enable the development of more efficient PTT agents for cancer or tumor therapy.
KW - cancer therapy
KW - conjugated polymers
KW - near-infrared absorption
KW - organic nanoparticles
KW - photothermal therapy
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U2 - 10.1002/smll.201402092
DO - 10.1002/smll.201402092
M3 - Article
C2 - 25367500
AN - SCOPUS:84926429665
SN - 1613-6810
VL - 11
SP - 1603
EP - 1610
JO - Small
JF - Small
IS - 13
ER -