Biochemical identification of glycosylation inhibiting factor

Kenji Katamura, Makoto Iwata, Akio Mori, Kimishige Ishizaka

Research output: Contribution to journalArticle

Abstract

Rat monoclonal antibody against mouse glycosylation inhibiting factors was obtained, and radiolabeled glycosylation inhibiting factors from the mouse T-cell hybridoma, 231F1 cells, were purified by using the monoclonal antibody and antilipomodulin antibody. Analysis of the affinity-purified lymphokine by PAGE demonstrated two proteins of 14.4 kDa and 41 kDa. Both proteins migrated similarly under the reduced and unreduced conditions, indicating that each of the two species consist of a single polypeptide chain. Biologic activity of the lymphokine was recovered by extraction of the proteins from gel slices followed by renaturation. Evidence was obtained that suggested the 14.4-kDa peptide was a degradation product of the 41-kDa molecules. The 14.4-kDa peptide was also recovered by extraction of the surface-labeled 231F1 cells with Ca2+ chelator, indicating that the glycosylation inhibiting factor is associated with the cell surface.

Original languageEnglish (US)
Pages (from-to)1903-1907
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number5
StatePublished - Mar 1990

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Lymphokines
Peptides
Monoclonal Antibodies
Proteins
Hybridomas
Chelating Agents
Gels
T-Lymphocytes
Antibodies
immunoglobulin-binding factors

Keywords

  • Lipocortin
  • Phospholipase inhibitory protein
  • Suppressor T-cell factor

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Biochemical identification of glycosylation inhibiting factor. / Katamura, Kenji; Iwata, Makoto; Mori, Akio; Ishizaka, Kimishige.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 5, 03.1990, p. 1903-1907.

Research output: Contribution to journalArticle

Katamura, Kenji ; Iwata, Makoto ; Mori, Akio ; Ishizaka, Kimishige. / Biochemical identification of glycosylation inhibiting factor. In: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Vol. 87, No. 5. pp. 1903-1907.
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