Biochemical Characterization of Myelin Isolated from the Central Nervous System of Xenopus Tadpoles

Bruce D. Trapp, Laurence J. McIntyre, Richard H. Quarles, Genïchro Nonaka, Ann Moser, Hugo W. Maser, Henry de F. Webster

Research output: Contribution to journalArticle

Abstract

Abstract: Myelin isolated from the central nervous system of Xenopus tadpoles was characterized biochemically and compared with Xenopus frog and mammalian myelins. Xenopus tadpole myelin contains the characteristic protein and lipid components of mammalian myelin, although quantitative differences exist. The biochemical composition of Xenopus tadpole myelin suggests that it is an immature form of XePnopus frog myelin. Basic protein and proteolipid protein are prominent components of Xenopus myelin, but isolated tadpole myelin contains a greater proportion of higher molecular weight proteins than Xenopus frog or mature mammalian myelin. The basic protein has a higher apparent molecular weight than mammalian myelin basic protein. The levels of 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase are significantly higher in whole tadpole brain homogenate and purified myelin than in similar mammalian preparations. Tadpole myelin lipids contain a higher proportion of phospholipids and less galactolipid than mammalian myelin. Tadpole myelin galactolipids include a high (16%) percentage of monogalactosyl diglyceride, a component found in only trace quantities (0.9%) in bovine myelin.

Original languageEnglish (US)
Pages (from-to)1241-1246
Number of pages6
JournalJournal of Neurochemistry
Volume34
Issue number5
DOIs
StatePublished - May 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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