Biochemical and quantitative analysis of Tamm Horsfall protein in rats

Jayashree A. Gokhale, Patricia A. Glenton, Saeed R. Khan

Research output: Contribution to journalArticlepeer-review

Abstract

The involvement of Tamm Horsfall protein (THP) in nephrolithiasis is currently under investigation in several laboratories. Although rat is a commonly used species as an in vivo model for such studies, there is only limited information available about the biochemical properties and excretion profile of THP in normal rats. In order to characterize rat THP, we purified and analyzed normal male rat THP, and compared it with normal human male urinary THP by gel electrophoresis. Both THPs migrated at approximately 90 KDa. and stained similarly for protein (Coomassie blue) as well as carbohydrates (periodic acid Schiff reagent). Compositional analysis revealed that rat THP was largely similar to human THP in amine acid and carbohydrate contents but showed differences in the individual sugar components from other mammals. There was considerable variation in the day-to-day urinary excretion of THP in normal rats, with values ranging from 552.96 μg to 2865.60 μg and a mean value of 1679.54 μg per 24 h. It was concluded from this study that rat THP did not contain any unusual biochemical components and was primarily similar to human THP in composition and mean urinary concentration.

Original languageEnglish (US)
Pages (from-to)347-354
Number of pages8
JournalUrological Research
Volume25
Issue number5
DOIs
StatePublished - Oct 1997
Externally publishedYes

Keywords

  • Amine acids
  • Carbohydrates
  • Gel electrophoresis
  • Sprague-Dawley rats
  • Tatum Hersfall protein

ASJC Scopus subject areas

  • Urology

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