Biochemical analysis of triggering signals induced by bridging of IgE receptors

T. Ishizaka

Research output: Contribution to journalArticle

Abstract

Bridging of IgE receptors on rat mast cell plasma membranes induces phospholipid methylation and a monophasic increase in cyclic AMP. The stimulation of phospholipid methylation in the plasma membrane appears to be intrinsic to the processes leading to Ca2+ influx and histamine release. Evidence was obtained that IgE receptors are closely associated with methyltransferases and adenylate cyclase in the plasma membranes. The activation of one enzyme is regulated by the other. An increase in the cyclic AMP level before receptor bridging suppressed phospholipid methylation. On the other hand, inhibition of phospholipid methylation may affect the initial rise in cyclic AMP. Our experiments also indicated that bridging the receptor activates a membrane-associated proteolytic enzyme. Inasmuch as the inhibition of the enzyme activation results in the suppression of both phospholipid methylation and initial rise in cyclic AMP induced by receptor bridging, the proteolytic enzyme may be involved in the activation of methyltransferases and adenylate cyclase.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalFederation proceedings
Volume41
Issue number1
StatePublished - 1982

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IgE Receptors
Methylation
Phospholipids
Cell Membrane
Cyclic AMP
Enzyme Activation
Methyltransferases
Adenylyl Cyclases
Peptide Hydrolases
Cyclic AMP Receptors
Histamine Release
Mast Cells
Membranes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Biochemical analysis of triggering signals induced by bridging of IgE receptors. / Ishizaka, T.

In: Federation proceedings, Vol. 41, No. 1, 1982, p. 17-21.

Research output: Contribution to journalArticle

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