Biochemical analysis of naturally processed antigenic peptides presented by mhc class i molecules

Takayuki Kanaseki, Nilabh Shastri

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Immune surveillance of infected or tumor cells by CD8+ T cells requires that MHC class I molecules present a diverse repertoire of peptides on the cell surface. Even a few copies of individual peptides among this mixture are suf fi cient for recognition by the antigen receptors of appropriate CD8+ T cells. Here we describe a method for biochemical analysis of the naturally processed peptides in living cells. The peptide mixture in cell extracts is fractionated using reverse phase high performance liquid chromatography and detected by the activation of CD8+ T cell hybridomas. The results provide information on the structure and amount of the peptides and yield insights into the mechanisms that generate the naturally processed peptides.

Original languageEnglish (US)
Title of host publicationAntigen Processing
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages179-185
Number of pages7
ISBN (Print)9781627032179
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume960
ISSN (Print)1064-3745

Keywords

  • Antigen presentation
  • Antigen processing
  • MHC

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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