Bioanalysis of N-acetyl-aspartyl-glutamate as a marker of glutamate carboxypeptidase II inhibition

Ajit G. Thomas; Camilo J. Rojas; Jeanette R. Hill; Michael Shaw; Barbara S. Slusher

doi:10.1016/j.ab.2010.04.029

Bioanalysis of N-acetyl-aspartyl-glutamate as a marker of glutamate carboxypeptidase II inhibition

Ajit G. Thomas, Camilo J. Rojas, Jeanette R. Hill, Michael Shaw, Barbara S. Slusher

School of Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We report the characterization of two methods for the analysis of N-acetyl-aspartyl-glutamate (NAAG) in biological fluids. In the first method, NAAG concentrations were calculated based on differences between glutamate concentrations before and after NAAG hydrolysis with exogenous glutamate carboxypeptidase II (GCP II) using high-performance liquid chromatography (HPLC) followed by fluorescence detection. In the second method, NAAG levels were quantified directly using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analyses of NAAG levels in human cerebrospinal fluid samples using either method gave similar results within experimental error, confirming the validity of the two independent measurements. These methods will be useful in future clinical trials to assess drug-induced GCP II inhibition in biological matrices.

Original language	English (US)
Pages (from-to)	94-96
Number of pages	3
Journal	Analytical biochemistry
Volume	404
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2010.04.029
State	Published - Sep 2010

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.ab.2010.04.029

Cite this

@article{2f86fb81f06b463996692cb3f3364b94,

title = "Bioanalysis of N-acetyl-aspartyl-glutamate as a marker of glutamate carboxypeptidase II inhibition",

abstract = "We report the characterization of two methods for the analysis of N-acetyl-aspartyl-glutamate (NAAG) in biological fluids. In the first method, NAAG concentrations were calculated based on differences between glutamate concentrations before and after NAAG hydrolysis with exogenous glutamate carboxypeptidase II (GCP II) using high-performance liquid chromatography (HPLC) followed by fluorescence detection. In the second method, NAAG levels were quantified directly using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analyses of NAAG levels in human cerebrospinal fluid samples using either method gave similar results within experimental error, confirming the validity of the two independent measurements. These methods will be useful in future clinical trials to assess drug-induced GCP II inhibition in biological matrices.",

author = "Thomas, {Ajit G.} and Rojas, {Camilo J.} and Hill, {Jeanette R.} and Michael Shaw and Slusher, {Barbara S.}",

year = "2010",

month = sep,

doi = "10.1016/j.ab.2010.04.029",

language = "English (US)",

volume = "404",

pages = "94--96",

journal = "Analytical biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Bioanalysis of N-acetyl-aspartyl-glutamate as a marker of glutamate carboxypeptidase II inhibition

AU - Thomas, Ajit G.

AU - Rojas, Camilo J.

AU - Hill, Jeanette R.

AU - Shaw, Michael

AU - Slusher, Barbara S.

PY - 2010/9

Y1 - 2010/9

N2 - We report the characterization of two methods for the analysis of N-acetyl-aspartyl-glutamate (NAAG) in biological fluids. In the first method, NAAG concentrations were calculated based on differences between glutamate concentrations before and after NAAG hydrolysis with exogenous glutamate carboxypeptidase II (GCP II) using high-performance liquid chromatography (HPLC) followed by fluorescence detection. In the second method, NAAG levels were quantified directly using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analyses of NAAG levels in human cerebrospinal fluid samples using either method gave similar results within experimental error, confirming the validity of the two independent measurements. These methods will be useful in future clinical trials to assess drug-induced GCP II inhibition in biological matrices.

AB - We report the characterization of two methods for the analysis of N-acetyl-aspartyl-glutamate (NAAG) in biological fluids. In the first method, NAAG concentrations were calculated based on differences between glutamate concentrations before and after NAAG hydrolysis with exogenous glutamate carboxypeptidase II (GCP II) using high-performance liquid chromatography (HPLC) followed by fluorescence detection. In the second method, NAAG levels were quantified directly using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analyses of NAAG levels in human cerebrospinal fluid samples using either method gave similar results within experimental error, confirming the validity of the two independent measurements. These methods will be useful in future clinical trials to assess drug-induced GCP II inhibition in biological matrices.

UR - http://www.scopus.com/inward/record.url?scp=77954089155&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954089155&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2010.04.029

DO - 10.1016/j.ab.2010.04.029

M3 - Article

C2 - 20434427

AN - SCOPUS:77954089155

SN - 0003-2697

VL - 404

SP - 94

EP - 96

JO - Analytical biochemistry

JF - Analytical biochemistry

IS - 1

ER -

Bioanalysis of N-acetyl-aspartyl-glutamate as a marker of glutamate carboxypeptidase II inhibition

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this