Bioactivity profiling with parallel mass spectrometry reveals an assemblage of green tea metabolites affording protection against human huntingtin and α-synuclein toxicity

Russell B. Williams, Will R. Gutekunst, P. Matthew Joyner, Wenzhen Duan, Qing Li, Christopher A. Ross, Todd D. Williams, Robert H. Cichewicz

Research output: Contribution to journalArticlepeer-review

Abstract

Aberrant protein aggregation and misfolding are key pathological features of many neurodegenerative disorders, including Huntington's and Parkinson's diseases. Compounds that offer protection from toxicity associated with aggregation-prone neurodegenerative proteins may have applications for the treatment of a multitude of disorders. A high-throughput bioassay system with parallel electrospray ionization mass spectrometry screening has been designed for critical evaluation of milligram quantities of natural product extracts, including dietary substances, for compounds of pharmacological relevance to the treatment of human neurodegenerative diseases. Using Saccharomyces cerevisiae strains engineered to express mutant human huntingtin and α-synuclein, we are able to identify extracts and compounds that protect cells from toxicity associated with these proteins. Applying this screening paradigm, we determined that a bioactive green tea extract contains an assemblage of catechins that were individually characterized for their respective protective effects against huntingtin and α-synuclein toxicity.

Original languageEnglish (US)
Pages (from-to)9450-9456
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume55
Issue number23
DOIs
StatePublished - Nov 14 2007

Keywords

  • Bioassay
  • Catechins
  • Huntingtin
  • Huntington's disease
  • Mass spectrometry
  • Natural products
  • Neuroprotective, green tea
  • Parkinson's disease
  • Protection
  • Protein aggregation
  • Saccharomyces cerevisiae
  • Yeast
  • α-synuclein

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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