Binding of thalidomide to α1-acid glycoprotein may be involved in its inhibition of tumor necrosis factor α production

Benjamin E. Turk, Hongsi Jiang, Jun Liu

Research output: Contribution to journalArticle

Abstract

In addition to its well known sedative and teratogenic effects, thalidomide also possesses potent immunomodulatory and antiinflammatory activities, being most effective against leprosy and chronic graft-versus- host disease. The immunomodulatory activity of thalidomide has been ascribed to the selective inhibition of tumor necrosis factor from monocytes. The molecular mechanism for the immunomodulatory effect of thalidomide remains unknown. To elucidate this mechanism, we synthesized an active photoaffinity label of thalidomide as a probe to identify the molecular target of the drug. Using the probe, we specifically labeled a pair of proteins of 43-45 kDa with high acidity from bovine thymus extract. Purification of these proteins and partial peptide sequence determination revealed them to be α1-acid glycoprotein (AGP). We show that the binding of thalidomide photoaffinity label to authentic human AGP is competed with both thalidomide and the nonradioactive photoaffinity label at concentrations comparable to those required for inhibition of production of tumor necrosis factor α from human monocytes, suggesting that AGP may be involved in the immunomodulatory activity of thalidomide.

Original languageEnglish (US)
Pages (from-to)7552-7556
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number15
DOIs
StatePublished - Jul 23 1996
Externally publishedYes

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Thalidomide
Glycoproteins
Tumor Necrosis Factor-alpha
Photoaffinity Labels
Acids
Monocytes
Thymus Extracts
Protein Sequence Analysis
Graft vs Host Disease
Leprosy
Hypnotics and Sedatives
Proteins
Anti-Inflammatory Agents
Pharmaceutical Preparations

Keywords

  • immunosuppression
  • monocytes
  • photoaffinity label

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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title = "Binding of thalidomide to α1-acid glycoprotein may be involved in its inhibition of tumor necrosis factor α production",
abstract = "In addition to its well known sedative and teratogenic effects, thalidomide also possesses potent immunomodulatory and antiinflammatory activities, being most effective against leprosy and chronic graft-versus- host disease. The immunomodulatory activity of thalidomide has been ascribed to the selective inhibition of tumor necrosis factor from monocytes. The molecular mechanism for the immunomodulatory effect of thalidomide remains unknown. To elucidate this mechanism, we synthesized an active photoaffinity label of thalidomide as a probe to identify the molecular target of the drug. Using the probe, we specifically labeled a pair of proteins of 43-45 kDa with high acidity from bovine thymus extract. Purification of these proteins and partial peptide sequence determination revealed them to be α1-acid glycoprotein (AGP). We show that the binding of thalidomide photoaffinity label to authentic human AGP is competed with both thalidomide and the nonradioactive photoaffinity label at concentrations comparable to those required for inhibition of production of tumor necrosis factor α from human monocytes, suggesting that AGP may be involved in the immunomodulatory activity of thalidomide.",
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author = "Turk, {Benjamin E.} and Hongsi Jiang and Jun Liu",
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AU - Turk, Benjamin E.

AU - Jiang, Hongsi

AU - Liu, Jun

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Y1 - 1996/7/23

N2 - In addition to its well known sedative and teratogenic effects, thalidomide also possesses potent immunomodulatory and antiinflammatory activities, being most effective against leprosy and chronic graft-versus- host disease. The immunomodulatory activity of thalidomide has been ascribed to the selective inhibition of tumor necrosis factor from monocytes. The molecular mechanism for the immunomodulatory effect of thalidomide remains unknown. To elucidate this mechanism, we synthesized an active photoaffinity label of thalidomide as a probe to identify the molecular target of the drug. Using the probe, we specifically labeled a pair of proteins of 43-45 kDa with high acidity from bovine thymus extract. Purification of these proteins and partial peptide sequence determination revealed them to be α1-acid glycoprotein (AGP). We show that the binding of thalidomide photoaffinity label to authentic human AGP is competed with both thalidomide and the nonradioactive photoaffinity label at concentrations comparable to those required for inhibition of production of tumor necrosis factor α from human monocytes, suggesting that AGP may be involved in the immunomodulatory activity of thalidomide.

AB - In addition to its well known sedative and teratogenic effects, thalidomide also possesses potent immunomodulatory and antiinflammatory activities, being most effective against leprosy and chronic graft-versus- host disease. The immunomodulatory activity of thalidomide has been ascribed to the selective inhibition of tumor necrosis factor from monocytes. The molecular mechanism for the immunomodulatory effect of thalidomide remains unknown. To elucidate this mechanism, we synthesized an active photoaffinity label of thalidomide as a probe to identify the molecular target of the drug. Using the probe, we specifically labeled a pair of proteins of 43-45 kDa with high acidity from bovine thymus extract. Purification of these proteins and partial peptide sequence determination revealed them to be α1-acid glycoprotein (AGP). We show that the binding of thalidomide photoaffinity label to authentic human AGP is competed with both thalidomide and the nonradioactive photoaffinity label at concentrations comparable to those required for inhibition of production of tumor necrosis factor α from human monocytes, suggesting that AGP may be involved in the immunomodulatory activity of thalidomide.

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